Diagnosis of hiv infection with instrument-free assays as an alternative to the elisa and western blot testing strategy: An evaluation in central africa

Frieda Behets, Kagoyire Bishagara, A. Disasi, Suzanne Likin, Robert W. Ryder, Christopher Brown, Thomas C. Quinn

Research output: Contribution to journalArticle

Abstract

The efficiency of an alternative instrument-free testing strategy was evaluated using a membrane-based rapid screening assay (HIVCHEK and its new version HIVCHEK1 +2) in serial combination with a particle agglutination assay (SERODIA-HIV). Among 1, 054 Zairian individuals at high risk of HIV infection, 573 were Western blot-positive for HIV-1 (54.4%) and none were Western blot-positive for HIV-2. In this group, the sensitivities of the serial combination HIVCHEK plus SERODIA-HIV and HIVCHEK 1 +2 plus SERODIA-HIV were 98.1 and 98.2%, respectively, and the specificities were 99.6 and 99.5% compared with HIV-1 Western blot. The positive predictive values were 99.6% for HIVCHEK plus SERODIA-HIV and 99.5% for HIVCHEK1 +2 plus SERODIA-HIV; the negative predictive values were 97.8 and 97.9%, respectively. Among 1, 495 pregnant women, 90 were Western blot-positive for HIV-1 (6.0%), and 54 of 1, 510 blood donors were HIV-1 Western blot-positive (3.6%). None were positive for HIV-2. The sensitivities of HIVCHEK plus SERODIA-HIV and HIVCHEK 1 +2 plus SERODIA-HIV in these groups were 98.6 and 99.3%, respectively, and the specificities were 99.8 and 99.7%. The positive and negative predictive values of HIVCHEK plus SERODIA-HIV were 96.6 and 99.9%, respectively, and they were 94.1 and 99.9%, respectively, for HIVCHEK1+2 plus SERODIA-HIV. These instrument-free testing strategies are efficient alternatives for serodiagnosis of HIV-1 infection, although their cost should be further reduced.

Original languageEnglish (US)
Pages (from-to)878-882
Number of pages5
JournalJournal of acquired immune deficiency syndromes
Volume5
Issue number9
StatePublished - Sep 1992
Externally publishedYes

Keywords

  • HIV
  • Instrument free assays
  • Serodiagnosis
  • Zaire

ASJC Scopus subject areas

  • Infectious Diseases
  • Pharmacology (medical)

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