Diagnosis of chlamydia trachomatis urethral infection in symptomatic and asymptomatic men by testing first-void urine in a ligase chain reaction assay

Max A. Chernesky, Helen Lee, Julius Schachter, John D. Burczak, Walter E. Stamm, William M. Mc cormack, Thomas C Quinn

Research output: Contribution to journalArticle

Abstract

A multicenter study compared ligase chain reaction (LCR) of Chlamydia trachomatis plasmid DNA with culture of urethral swab specimens from 542 men (study A); a second study (B) compared LCR of first-void urine (FVU) with urethral swab cultures from 1043 men. Discordant results were resolved with direct fluorescent antibody staining of sediments from the FVU or urethral culture specimen and with a second LCR directed against a fragment of the major outer membrane protein gene. Test performance was calculated on the basis of an expanded reference standard. The LCR plasmid assay had a sensitivity of 98.0% in study A and 93.5% in study B; specificity was 99.8%-100%. The sensitivity of culturing urethral swabs from all study sites was 68.2% (range by sites, 40.0%-84.6%). The presence or absence of urethral symptoms did not alter the results. Use of this LCR test should allow more meaningful investigation and treatment of C. trachomatis infections in men.

Original languageEnglish (US)
Pages (from-to)1308-1311
Number of pages4
JournalJournal of Infectious Diseases
Volume170
Issue number5
DOIs
StatePublished - 1994

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Ligase Chain Reaction
Chlamydia trachomatis
Urine
Infection
Plasmids
Chlamydia Infections
Multicenter Studies
Membrane Proteins
Staining and Labeling
Antibodies
DNA
Genes

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Diagnosis of chlamydia trachomatis urethral infection in symptomatic and asymptomatic men by testing first-void urine in a ligase chain reaction assay. / Chernesky, Max A.; Lee, Helen; Schachter, Julius; Burczak, John D.; Stamm, Walter E.; Mc cormack, William M.; Quinn, Thomas C.

In: Journal of Infectious Diseases, Vol. 170, No. 5, 1994, p. 1308-1311.

Research output: Contribution to journalArticle

Chernesky, Max A. ; Lee, Helen ; Schachter, Julius ; Burczak, John D. ; Stamm, Walter E. ; Mc cormack, William M. ; Quinn, Thomas C. / Diagnosis of chlamydia trachomatis urethral infection in symptomatic and asymptomatic men by testing first-void urine in a ligase chain reaction assay. In: Journal of Infectious Diseases. 1994 ; Vol. 170, No. 5. pp. 1308-1311.
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