O-Linked β-N-acetylglucosamine modification (O-GlcNAcylation) of proteins is a dynamic posttranslational modification which occurs in the nucleus and the cytoplasm. Increase of O-GlcNAcylation is involved in the glucose toxicity and chronic hyperglycemia-induced insulin resistance, a major hallmark of type 2 diabetes. O-GlcNAcylation is regulated by the concerted actions of O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). Studies on the overexpression of OGT and knockdown of OGA demonstrate that elevation of O-GlcNAcylation through the hexosamine biosynthetic pathway plays an important role in the development of glucose resistance and diabetic complications. This chapter overviews the role of O-GlcNAc in the type 2 diabetes and diabetic complications and the methods of analysis of O-GlcNAcylation in diabetes.
- Insulin resistance
- O-GlcNAc transferase
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)