Abstract
The palmitoyl acyltransferase (PAT) DHHC8 is implicated in synaptic regulation but few DHHC8 substrates are known. Here we report that DHHC8 binds and palmitoylates the PDZ domain-containing protein PICK1 at a cysteine residue that is essential for long-term synaptic depression (LTD) in cultured mouse cerebellar Purkinje neurons. Cerebellar LTD is palmitoylation-dependent and induction of LTD requires DHHC8. Furthermore, PICK1 is a critical DHHC8 substrate whose palmitoylation is necessary for LTD. These results identify the first DHHC8 substrate required for a specific form of synaptic plasticity and provide new insights into synaptic roles of palmitoylation.
Original language | English (US) |
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Pages (from-to) | 15401-15407 |
Number of pages | 7 |
Journal | Journal of Neuroscience |
Volume | 33 |
Issue number | 39 |
DOIs | |
State | Published - 2013 |
ASJC Scopus subject areas
- General Neuroscience