Dexamethasone affects cytokine-mediated adhesion of HL-60 human promyelocytic leukemia cells to cultured dermal microvascular endothelial cells

Nilesh A. Patel, Jitesh A. Patel, Monique F. Stins, Kwang S. Kim, Sulie L. Chang

Research output: Contribution to journalArticlepeer-review

Abstract

Leukocyte endothelial adhesion (LEA) is the prelude to a complex cascade of reactions following an immunological challenge. Recently, LEA has been implicated in the molecular basis of several dermatological disorders. While the role of proinflammatory cytokines, such as interleukin-1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α), in LEA has been investigated using nondermal models, limited data exist regarding their effects on LEA in dermal models. This study shows that cotreatment of cultured human dermal endothelial cells (CADMEC) with IL-1β and TNF-α resulted in a marked increase in the adherence of human promyelocytic leukemia (HL-60) cells to CAD. MEC and an increase in expression of intercellular adhesion molecule-1 and E-selectin. Pretreatment of CADMEC with dexamethasone, a long-lasting glucocorticoid, resulted in a decrease in both HL-60 cell adhesion to CADMEC and adhesion molecule expression. Taken together, these data demonstrate that LEA may play a role in inflammatory skin conditions and in the mechanisms underlying the potential use of glucocorticoids as a treatment option.

Original languageEnglish (US)
Pages (from-to)387-394
Number of pages8
JournalClinical Immunology
Volume99
Issue number3
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Dexamethasone
  • IL-1β
  • LEA
  • TNF-α

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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