Developmental role of GnRH and PACAP in a zebrafish model

Nancy M. Sherwood; Sheng Wu

doi:10.1016/j.ygcen.2005.02.007

Developmental role of GnRH and PACAP in a zebrafish model

Nancy M. Sherwood, Sheng Wu

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

GnRH is expressed early in development long before reproduction begins. To determine whether GnRH has a role in development, gene knockdown with morpholinos was used in one-cell zebrafish embryos to block translation of gnrh mRNA into protein. Gene knockdown of gnrh2, gnrh3 or both at the one-cell stage resulted in a high percentage of embryos at 24-48 h with a defective mid-hindbrain boundary and underdeveloped eyes; a small percentage of embryos at 72 h had a defective heart. In similar studies on GHRH-PACAP, gene knockdown resulted in a smaller brain and eyes, but a normal-appearing heart. The evidence supports a role for the three neuropeptides in early development.

Original language	English (US)
Pages (from-to)	74-80
Number of pages	7
Journal	General and Comparative Endocrinology
Volume	142
Issue number	1-2 SPEC. ISS.
DOIs	https://doi.org/10.1016/j.ygcen.2005.02.007
State	Published - May 15 2005
Externally published	Yes

Keywords

Brain
Development
Fish
GHRH
Gene knockdown
GnRH
Morpholino
Neuropeptides
PACAP
Reproduction
Zebrafish

ASJC Scopus subject areas

Animal Science and Zoology
Endocrinology

Access to Document

10.1016/j.ygcen.2005.02.007

Cite this

@article{30aac234c229440f91891edda642d769,

title = "Developmental role of GnRH and PACAP in a zebrafish model",

abstract = "GnRH is expressed early in development long before reproduction begins. To determine whether GnRH has a role in development, gene knockdown with morpholinos was used in one-cell zebrafish embryos to block translation of gnrh mRNA into protein. Gene knockdown of gnrh2, gnrh3 or both at the one-cell stage resulted in a high percentage of embryos at 24-48 h with a defective mid-hindbrain boundary and underdeveloped eyes; a small percentage of embryos at 72 h had a defective heart. In similar studies on GHRH-PACAP, gene knockdown resulted in a smaller brain and eyes, but a normal-appearing heart. The evidence supports a role for the three neuropeptides in early development.",

keywords = "Brain, Development, Fish, GHRH, Gene knockdown, GnRH, Morpholino, Neuropeptides, PACAP, Reproduction, Zebrafish",

author = "Sherwood, {Nancy M.} and Sheng Wu",

note = "Funding Information: We thank Bruce Adams, Erica Fradinger, Kataaki Okubo, and Yoshitaka Nagahama for allowing us to cite unpublished work. Canadian Natural Sciences and Engineering Research Council (NSERC) supported our research.",

year = "2005",

month = may,

day = "15",

doi = "10.1016/j.ygcen.2005.02.007",

language = "English (US)",

volume = "142",

pages = "74--80",

journal = "General and Comparative Endocrinology",

issn = "0016-6480",

publisher = "Academic Press Inc.",

number = "1-2 SPEC. ISS.",

}

TY - JOUR

T1 - Developmental role of GnRH and PACAP in a zebrafish model

AU - Sherwood, Nancy M.

AU - Wu, Sheng

N1 - Funding Information: We thank Bruce Adams, Erica Fradinger, Kataaki Okubo, and Yoshitaka Nagahama for allowing us to cite unpublished work. Canadian Natural Sciences and Engineering Research Council (NSERC) supported our research.

PY - 2005/5/15

Y1 - 2005/5/15

N2 - GnRH is expressed early in development long before reproduction begins. To determine whether GnRH has a role in development, gene knockdown with morpholinos was used in one-cell zebrafish embryos to block translation of gnrh mRNA into protein. Gene knockdown of gnrh2, gnrh3 or both at the one-cell stage resulted in a high percentage of embryos at 24-48 h with a defective mid-hindbrain boundary and underdeveloped eyes; a small percentage of embryos at 72 h had a defective heart. In similar studies on GHRH-PACAP, gene knockdown resulted in a smaller brain and eyes, but a normal-appearing heart. The evidence supports a role for the three neuropeptides in early development.

AB - GnRH is expressed early in development long before reproduction begins. To determine whether GnRH has a role in development, gene knockdown with morpholinos was used in one-cell zebrafish embryos to block translation of gnrh mRNA into protein. Gene knockdown of gnrh2, gnrh3 or both at the one-cell stage resulted in a high percentage of embryos at 24-48 h with a defective mid-hindbrain boundary and underdeveloped eyes; a small percentage of embryos at 72 h had a defective heart. In similar studies on GHRH-PACAP, gene knockdown resulted in a smaller brain and eyes, but a normal-appearing heart. The evidence supports a role for the three neuropeptides in early development.

KW - Brain

KW - Development

KW - Fish

KW - GHRH

KW - Gene knockdown

KW - GnRH

KW - Morpholino

KW - Neuropeptides

KW - PACAP

KW - Reproduction

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=18144407228&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=18144407228&partnerID=8YFLogxK

U2 - 10.1016/j.ygcen.2005.02.007

DO - 10.1016/j.ygcen.2005.02.007

M3 - Article

C2 - 15862551

AN - SCOPUS:18144407228

SN - 0016-6480

VL - 142

SP - 74

EP - 80

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

IS - 1-2 SPEC. ISS.

ER -

Developmental role of GnRH and PACAP in a zebrafish model

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this