Developmental regulation of TCRδ locus accessibility and expression by the TCRδ enhancer

Robert J. Monroe, Barry P. Sleckman, Brianna C. Monroe, Bernard Khor, Steven Claypool, Roger Ferrini, Laurie Davidson, Frederick W. Alt

Research output: Contribution to journalArticlepeer-review

Abstract

We have used gene-targeted mutation to assess the role of the T cell receptor δ (TCRδ) enhancer (Eδ) in αβ and γδ T cell development. Mice lacking Eδ exhibited no defects in αβ T cell development but had a severe reduction in thymic and peripheral γδ T cells and decreased VDJδ rearrangements. Simultaneous deletion of both Eδ and the TCRα enhancer (Eα) demonstrated that residual TCRδ rearrangements were not driven by Eα, implicating additional elements in TCRδ locus accessibility. Surprisingly, while deletion of Eδ severely impaired germline TCRδ expression in double- negative thymocytes, absence of Eδ did not affect expression of mature δ transcripts in γδ T cells. We conclude that Eδ has an important role in TCRδ locus regulation at early, but not late, stages of γδ T cell development.

Original languageEnglish (US)
Pages (from-to)503-513
Number of pages11
JournalImmunity
Volume10
Issue number5
DOIs
StatePublished - May 1999
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Developmental regulation of TCRδ locus accessibility and expression by the TCRδ enhancer'. Together they form a unique fingerprint.

Cite this