Developmental expression of eNOS in postnatal swine mesenteric artery

Kristina M. Reber, Baogen Y. Su, K. Reed Clark, Dana L. Pohlman, Charles E. Miller, Philip T. Nowicki

Research output: Contribution to journalArticle

Abstract

Developmental changes in the expression of endothelial nitric oxide synthase (eNOS) within the mesenteric artery of swine were studied in fetal (110 days postconception/ 117 days total gestation) and on postnatal days 1, 3, 10, and 30. Subjects in the 1-day-old group were subdivided into fed and nonfed. Transcription of eNOS was determined by realtime PCR, protein expression was evaluated by Western blotting, and hemodynamic and oxygenation parameters were measured within in situ gut loops before and after the administration of NG-monomethyl-L-arginine (L-NMMA). The abundance of eNOS mRNA remained steady throughout all ages. In contrast, expression of eNOS protein was twofold greater in the 1-day-old fed subjects compared with fetal or 1-day-old nonfed subjects. eNOS protein expression remained elevated on day 3, increased on day 10, and then declined to a level similar to the day 1 nonfed group by postnatal day 30. Intestinal vascular resistance was 31% lower in the day 1 fed group when compared to the day 1 nonfed group; resistance continued to decline through day 10 but then significantly increased on day 30. We conclude that the expression of eNOS changes within the mesenteric artery during early postnatal development at a posttranscriptional level.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume283
Issue number6 46-6
StatePublished - Dec 1 2002
Externally publishedYes

Fingerprint

Mesenteric Arteries
Nitric Oxide Synthase Type III
Swine
omega-N-Methylarginine
Proteins
Vascular Resistance
Hemodynamics
Western Blotting
Pregnancy
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Necrotizing enterocolitis
  • Newborn intestine

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology

Cite this

Reber, K. M., Su, B. Y., Reed Clark, K., Pohlman, D. L., Miller, C. E., & Nowicki, P. T. (2002). Developmental expression of eNOS in postnatal swine mesenteric artery. American Journal of Physiology - Gastrointestinal and Liver Physiology, 283(6 46-6).

Developmental expression of eNOS in postnatal swine mesenteric artery. / Reber, Kristina M.; Su, Baogen Y.; Reed Clark, K.; Pohlman, Dana L.; Miller, Charles E.; Nowicki, Philip T.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 283, No. 6 46-6, 01.12.2002.

Research output: Contribution to journalArticle

Reber, KM, Su, BY, Reed Clark, K, Pohlman, DL, Miller, CE & Nowicki, PT 2002, 'Developmental expression of eNOS in postnatal swine mesenteric artery', American Journal of Physiology - Gastrointestinal and Liver Physiology, vol. 283, no. 6 46-6.
Reber KM, Su BY, Reed Clark K, Pohlman DL, Miller CE, Nowicki PT. Developmental expression of eNOS in postnatal swine mesenteric artery. American Journal of Physiology - Gastrointestinal and Liver Physiology. 2002 Dec 1;283(6 46-6).
Reber, Kristina M. ; Su, Baogen Y. ; Reed Clark, K. ; Pohlman, Dana L. ; Miller, Charles E. ; Nowicki, Philip T. / Developmental expression of eNOS in postnatal swine mesenteric artery. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2002 ; Vol. 283, No. 6 46-6.
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