Developmental expression of endothelin receptors in postnatal swine mesenteric artery

Baogen Y. Su; Kristina M. Reber; Craig A. Nankervis

doi:10.1203/01.PDR.0000134253.86014.B9

Developmental expression of endothelin receptors in postnatal swine mesenteric artery

Baogen Y. Su, Kristina M. Reber, Craig A. Nankervis

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Studies were conducted to determine whether endothelin (ET) ET_A and ET_B receptor protein and mRNA expression is developmentally regulated in the postnatal swine mesenteric circulation. To this end, Western blotting and real-time reverse PCR were performed on protein and total RNA isolated from the mesenteric artery harvested from 3-, 10-, and 30-d-old swine. Western blot analysis revealed that ET_A and ET_B receptor protein expression in the swine mesenteric artery decreased over the age range studied; thus, ET_A and ET_B receptor protein expression was significantly greater in the 3-d-old group then progressively declined over the first postnatal month. Similar to the Western data, real-time PCR analysis revealed that ET_A and ET_B receptor mRNA expression also decreased over the age range studied; thus, ET_A and ET_B receptor mRNA expression was significantly greater in the 3-d-old group then progressively declined over the first postnatal month. Immunohistochemistry localized the ET_A receptor to the vascular smooth muscle and the ET_B receptor to the endothelial cell layer. Additionally, we report a partial cDNA sequence for the swine ET_B receptor. We conclude that ET_A and ET_B receptor protein and mRNA expression is developmentally regulated in the postnatal swine mesenteric artery, being expressed to a greater degree in younger animals.

Original language	English (US)
Pages (from-to)	359-365
Number of pages	7
Journal	Pediatric research
Volume	56
Issue number	3
DOIs	https://doi.org/10.1203/01.PDR.0000134253.86014.B9
State	Published - Sep 2004

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health

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title = "Developmental expression of endothelin receptors in postnatal swine mesenteric artery",

abstract = "Studies were conducted to determine whether endothelin (ET) ETA and ETB receptor protein and mRNA expression is developmentally regulated in the postnatal swine mesenteric circulation. To this end, Western blotting and real-time reverse PCR were performed on protein and total RNA isolated from the mesenteric artery harvested from 3-, 10-, and 30-d-old swine. Western blot analysis revealed that ETA and ETB receptor protein expression in the swine mesenteric artery decreased over the age range studied; thus, ETA and ETB receptor protein expression was significantly greater in the 3-d-old group then progressively declined over the first postnatal month. Similar to the Western data, real-time PCR analysis revealed that ETA and ETB receptor mRNA expression also decreased over the age range studied; thus, ETA and ETB receptor mRNA expression was significantly greater in the 3-d-old group then progressively declined over the first postnatal month. Immunohistochemistry localized the ETA receptor to the vascular smooth muscle and the ETB receptor to the endothelial cell layer. Additionally, we report a partial cDNA sequence for the swine ETB receptor. We conclude that ETA and ETB receptor protein and mRNA expression is developmentally regulated in the postnatal swine mesenteric artery, being expressed to a greater degree in younger animals.",

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AU - Nankervis, Craig A.

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AB - Studies were conducted to determine whether endothelin (ET) ETA and ETB receptor protein and mRNA expression is developmentally regulated in the postnatal swine mesenteric circulation. To this end, Western blotting and real-time reverse PCR were performed on protein and total RNA isolated from the mesenteric artery harvested from 3-, 10-, and 30-d-old swine. Western blot analysis revealed that ETA and ETB receptor protein expression in the swine mesenteric artery decreased over the age range studied; thus, ETA and ETB receptor protein expression was significantly greater in the 3-d-old group then progressively declined over the first postnatal month. Similar to the Western data, real-time PCR analysis revealed that ETA and ETB receptor mRNA expression also decreased over the age range studied; thus, ETA and ETB receptor mRNA expression was significantly greater in the 3-d-old group then progressively declined over the first postnatal month. Immunohistochemistry localized the ETA receptor to the vascular smooth muscle and the ETB receptor to the endothelial cell layer. Additionally, we report a partial cDNA sequence for the swine ETB receptor. We conclude that ETA and ETB receptor protein and mRNA expression is developmentally regulated in the postnatal swine mesenteric artery, being expressed to a greater degree in younger animals.

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Developmental expression of endothelin receptors in postnatal swine mesenteric artery

Abstract

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