Developmental expression of chemokine receptor genes in the human fetus

Wenge Lu; Jason A. Gersting; Akhil Maheshwari; Robert D. Christensen; Darlene A. Calhoun

doi:10.1016/j.earlhumdev.2004.10.022

Developmental expression of chemokine receptor genes in the human fetus

Wenge Lu, Jason A. Gersting, Akhil Maheshwari, Robert D. Christensen, Darlene A. Calhoun

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Background: Chemokines induce cell motility during embryogenesis by activating specific receptors. While the orchestration of organogenesis is complex and requires the interaction of many morphoregulatory molecules that lead to coordinated organ development, limited knowledge exists regarding the human developmental biology of chemokines and their receptors. Such information on chemokine receptor expression could potentially enhance our understanding of organogenesis in the normal human fetus. Aim: To determine the distribution of the CXC receptors (CXCR-1, CXCR-2, CXCR-3, and CXCR-4) and SDF-1 in human fetuses. Subjects: Tissues from human fetuses 12-15 weeks (n=5) and 16-19 weeks (n=5) gestation were studied. Outcome measures: Reverse transcription-PCR was performed to simultaneously determine the gene expression of CXCR-1-4 and SDF-1, and immunohistochemical staining of non-hematopoietic tissues was used to determine the specific cellular proteins. Results: CXCR-1-4 and SDF-1 mRNA were present in every tissue examined. The expression of CXCR-3 in kidney, liver, and brain was dependent upon gestational age. CXCR-1-4 protein was expressed in non-hematopoietic cells in the brain, heart, intestine, and kidney. Conclusions: CXCR-1-4 and SDF-1 genes are widely expressed in the normal human fetus. This suggests that these gene products could influence fetal development.

Original language	English (US)
Pages (from-to)	489-496
Number of pages	8
Journal	Early Human Development
Volume	81
Issue number	6
DOIs	https://doi.org/10.1016/j.earlhumdev.2004.10.022
State	Published - Jun 2005
Externally published	Yes

Keywords

CXCR
Cell motility
Chemokines
Embryogenesis
SDF-1

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health
Obstetrics and Gynecology

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10.1016/j.earlhumdev.2004.10.022

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@article{4559728bd8ad4b9da493cf4bdb2e1be9,

title = "Developmental expression of chemokine receptor genes in the human fetus",

abstract = "Background: Chemokines induce cell motility during embryogenesis by activating specific receptors. While the orchestration of organogenesis is complex and requires the interaction of many morphoregulatory molecules that lead to coordinated organ development, limited knowledge exists regarding the human developmental biology of chemokines and their receptors. Such information on chemokine receptor expression could potentially enhance our understanding of organogenesis in the normal human fetus. Aim: To determine the distribution of the CXC receptors (CXCR-1, CXCR-2, CXCR-3, and CXCR-4) and SDF-1 in human fetuses. Subjects: Tissues from human fetuses 12-15 weeks (n=5) and 16-19 weeks (n=5) gestation were studied. Outcome measures: Reverse transcription-PCR was performed to simultaneously determine the gene expression of CXCR-1-4 and SDF-1, and immunohistochemical staining of non-hematopoietic tissues was used to determine the specific cellular proteins. Results: CXCR-1-4 and SDF-1 mRNA were present in every tissue examined. The expression of CXCR-3 in kidney, liver, and brain was dependent upon gestational age. CXCR-1-4 protein was expressed in non-hematopoietic cells in the brain, heart, intestine, and kidney. Conclusions: CXCR-1-4 and SDF-1 genes are widely expressed in the normal human fetus. This suggests that these gene products could influence fetal development.",

keywords = "CXCR, Cell motility, Chemokines, Embryogenesis, SDF-1",

author = "Wenge Lu and Gersting, {Jason A.} and Akhil Maheshwari and Christensen, {Robert D.} and Calhoun, {Darlene A.}",

note = "Funding Information: Supported by grants HL-44951 (RDC), RR-00083 (RDC), HD-01180 (DAC) and HD-42326 (DAC) from the National Institutes of Health. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2005",

month = jun,

doi = "10.1016/j.earlhumdev.2004.10.022",

language = "English (US)",

volume = "81",

pages = "489--496",

journal = "Early Human Development",

issn = "0378-3782",

publisher = "Elsevier Ireland Ltd",

number = "6",

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TY - JOUR

T1 - Developmental expression of chemokine receptor genes in the human fetus

AU - Lu, Wenge

AU - Gersting, Jason A.

AU - Maheshwari, Akhil

AU - Christensen, Robert D.

AU - Calhoun, Darlene A.

N1 - Funding Information: Supported by grants HL-44951 (RDC), RR-00083 (RDC), HD-01180 (DAC) and HD-42326 (DAC) from the National Institutes of Health. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2005/6

Y1 - 2005/6

N2 - Background: Chemokines induce cell motility during embryogenesis by activating specific receptors. While the orchestration of organogenesis is complex and requires the interaction of many morphoregulatory molecules that lead to coordinated organ development, limited knowledge exists regarding the human developmental biology of chemokines and their receptors. Such information on chemokine receptor expression could potentially enhance our understanding of organogenesis in the normal human fetus. Aim: To determine the distribution of the CXC receptors (CXCR-1, CXCR-2, CXCR-3, and CXCR-4) and SDF-1 in human fetuses. Subjects: Tissues from human fetuses 12-15 weeks (n=5) and 16-19 weeks (n=5) gestation were studied. Outcome measures: Reverse transcription-PCR was performed to simultaneously determine the gene expression of CXCR-1-4 and SDF-1, and immunohistochemical staining of non-hematopoietic tissues was used to determine the specific cellular proteins. Results: CXCR-1-4 and SDF-1 mRNA were present in every tissue examined. The expression of CXCR-3 in kidney, liver, and brain was dependent upon gestational age. CXCR-1-4 protein was expressed in non-hematopoietic cells in the brain, heart, intestine, and kidney. Conclusions: CXCR-1-4 and SDF-1 genes are widely expressed in the normal human fetus. This suggests that these gene products could influence fetal development.

AB - Background: Chemokines induce cell motility during embryogenesis by activating specific receptors. While the orchestration of organogenesis is complex and requires the interaction of many morphoregulatory molecules that lead to coordinated organ development, limited knowledge exists regarding the human developmental biology of chemokines and their receptors. Such information on chemokine receptor expression could potentially enhance our understanding of organogenesis in the normal human fetus. Aim: To determine the distribution of the CXC receptors (CXCR-1, CXCR-2, CXCR-3, and CXCR-4) and SDF-1 in human fetuses. Subjects: Tissues from human fetuses 12-15 weeks (n=5) and 16-19 weeks (n=5) gestation were studied. Outcome measures: Reverse transcription-PCR was performed to simultaneously determine the gene expression of CXCR-1-4 and SDF-1, and immunohistochemical staining of non-hematopoietic tissues was used to determine the specific cellular proteins. Results: CXCR-1-4 and SDF-1 mRNA were present in every tissue examined. The expression of CXCR-3 in kidney, liver, and brain was dependent upon gestational age. CXCR-1-4 protein was expressed in non-hematopoietic cells in the brain, heart, intestine, and kidney. Conclusions: CXCR-1-4 and SDF-1 genes are widely expressed in the normal human fetus. This suggests that these gene products could influence fetal development.

KW - CXCR

KW - Cell motility

KW - Chemokines

KW - Embryogenesis

KW - SDF-1

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JF - Early Human Development

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ER -

Developmental expression of chemokine receptor genes in the human fetus

Abstract

Keywords

ASJC Scopus subject areas

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