Abstract
Intramolecular crosslink of hemoglobin tetramers solved the problem of urine elimination and short intravascular retention time of cell free hemoglobin infusion. It also produced a family of crosslinked hemoglobins with P50 between 18 and 30 mmHg. However, it did not solve the problem of MAP increases in infused animals. It was proven that extravasation of hemoglobin into interstitial fluid was responsible for MAP increases. Extravasation and the MAP increase was avoided using a hemoglobin polymer with average size near 25 MDa. In spite of a very high oxygen affinity, this polymer delivered oxygen to tissues, producing either vasodilation or vasoconstriction according to oxygen needs. It was also proven that cell free hemoglobins are more efficient than red cells in delivering oxygen to tissues.
Original language | English (US) |
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Pages (from-to) | 11-18 |
Number of pages | 8 |
Journal | Artificial Cells, Blood Substitutes, and Biotechnology |
Volume | 35 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2007 |
Keywords
- Blood substitutes
- Crosslinking
- Extravasation
- Zero-link polymers
ASJC Scopus subject areas
- Biotechnology
- Biomedical Engineering