Development of transgenic fungi that kill human malaria parasites in mosquitoes

Weiguo Fang, Joel Vega-Rodríguez, Anil K. Ghosh, Marcelo Jacobs-Lorena, Angray Kang, Raymond J. St. Leger

Research output: Contribution to journalArticle

Abstract

Metarhizium anisopliae infects mosquitoes through the cuticle and proliferates in the hemolymph. To allow M. anisopliae to combat malaria in mosquitoes with advanced malaria infections, we produced recombinant strains expressing molecules that target sporozoites as they travel through the hemolymph to the salivary glands. Eleven days after a Plasmodium-infected blood meal, mosquitoes were treated with M. anisopliae expressing salivary gland and midgut peptide 1 (SM1), which blocks attachment of sporozoites to salivary glands; a single-chain antibody that agglutinates sporozoites; or scorpine, which is an antimicrobial toxin. These reduced sporozoite counts by 71%, 85%, and 90%, respectively. M. anisopliae expressing scorpine and an [SM1] 8:scorpine fusion protein reduced sporozoite counts by 98%, suggesting that Metarhizium-mediated inhibition of Plasmodium development could be a powerful weapon for combating malaria.

Original languageEnglish (US)
Pages (from-to)1074-1077
Number of pages4
JournalScience
Volume331
Issue number6020
DOIs
StatePublished - Feb 25 2011

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Development of transgenic fungi that kill human malaria parasites in mosquitoes'. Together they form a unique fingerprint.

  • Cite this

    Fang, W., Vega-Rodríguez, J., Ghosh, A. K., Jacobs-Lorena, M., Kang, A., & St. Leger, R. J. (2011). Development of transgenic fungi that kill human malaria parasites in mosquitoes. Science, 331(6020), 1074-1077. https://doi.org/10.1126/science.1199115