Development of the human choriocapillaris

Gerard Anthony Lutty, T. Hasegawa, T. Baba, R. Grebe, I. Bhutto, D. S. McLeod

Research output: Contribution to journalArticle

Abstract

Vasculogenesis and/or angiogenesis are thought to be the major mechanisms for new vessel formation during development. A third mechanism, haemo-vasculogenesis, has been described in which blood vessel and blood cells (haematopoiesis (expression of CD34+) and erythropoiesis (presence of chain ofε haemoglobin or Hb-ε+)) differentiate from a common precursor, the haemangioblast. This review describes the mechanism(s) for development of human choroidal vascular from 6 until 22 weeks gestation (WG). Endothelial cell or EC (CD31, CD34, CD39, VEGFR-2) and angioblast (CD39, VEGFR-2) markers were present in choriocapillaris (CC) by 7 WG through 22 WG. From 6 to 8 WG, many erythroblasts (nucleated Hb-ε+ RBCs) were observed in the CC layer. Erythroblasts (Hb-ε+) were also positive for CD34, CD31, and/or VEGFR-2. Proliferation of vascular cells (Ki67+), suggesting angiogenesis, was not observed until 12 WG. TEM analysis demonstrated that CC was structurally immature even at 11 WG: no basement membrane, absence of pericytes, and poorly formed lumens that were filled with filopodia. Contiguous fenestrations and significant PV-1 (protein in diaphragms of fenestrations) were not observed until 21-22 WG. Smooth muscle actin was prominent at 20 WG and the maturation of pericytes was confirmed by TEM. Therefore, the embryonic CC appears to form initially by haemo- vasculogenesis(Hb-ε+/CD31+cells), whereas angiogenesis (CD34+ /Ki67+) appears to be the mode of intermediate and large choroidal vessel development later in the foetus. Contiguous fenestrations, mature pericytes, and EC basal lamina occur late in development, around 22 WG, which coincides with photoreceptors developing inner segments.

Original languageEnglish (US)
Pages (from-to)408-415
Number of pages8
JournalEye (London, England)
Volume24
Issue number3
DOIs
StatePublished - Mar 2010

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Human Development
Pregnancy
Vascular Endothelial Growth Factor Receptor-2
Pericytes
Blood Vessels
Erythroblasts
Basement Membrane
Hemangioblasts
Pseudopodia
Erythropoiesis
Hematopoiesis
Diaphragm
Smooth Muscle
Actins
Blood Cells
Hemoglobins
Fetus
Endothelial Cells
Cell Proliferation

Keywords

  • Choriocapillaris
  • Fenestrations
  • Foetal
  • Haemo-vasculogenesis
  • Pericytes
  • Ultrastructure

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Development of the human choriocapillaris. / Lutty, Gerard Anthony; Hasegawa, T.; Baba, T.; Grebe, R.; Bhutto, I.; McLeod, D. S.

In: Eye (London, England), Vol. 24, No. 3, 03.2010, p. 408-415.

Research output: Contribution to journalArticle

Lutty, GA, Hasegawa, T, Baba, T, Grebe, R, Bhutto, I & McLeod, DS 2010, 'Development of the human choriocapillaris', Eye (London, England), vol. 24, no. 3, pp. 408-415. https://doi.org/10.1038/eye.2009.318
Lutty, Gerard Anthony ; Hasegawa, T. ; Baba, T. ; Grebe, R. ; Bhutto, I. ; McLeod, D. S. / Development of the human choriocapillaris. In: Eye (London, England). 2010 ; Vol. 24, No. 3. pp. 408-415.
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