Abstract
This study developed a reverse transcriptase-polymerase chain reaction (RT-PCR) detection system for enteric viruses in sample concentrates obtained by conventional filter adsorption-elution methods. One liter beef extract (BE)-glycine (G) eluant seeded with poliovirus 1 and hepatitis A virus (HAV) was used as a model system and the eluant further processed for RT-PCR compatibility. Sample concentration and purification procedures consisted of polyethylene glycol (PEG) precipitation, Pro-Cipitate (Affinity Technology, New Brunswick, NJ) precipitation, a second PEG precipitation, spin-column chromatography, and ultrafiltration. Sample volumes are reduced from 1 L to 20-40 μL and purified sufficiently for viral detection by RT-PCR. As little as 3 PFU of poliovirus 1 in an initial 1 L eluate were detected by RT-PCR.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 211-218 |
| Number of pages | 8 |
| Journal | Water Science and Technology |
| Volume | 27 |
| Issue number | 3-4 |
| DOIs | |
| State | Published - Jan 1 1993 |
| Externally published | Yes |
| Event | Proceedings of the 16th Biennial Conference and Exposition of the International Association on Water Pollution Research and Control - Washington, DC, USA Duration: May 24 1992 → May 30 1992 |
Keywords
- Beef extract
- Concentration
- Enteric viruses
- PEG precipitation
- Purification
- Rt-PCR
- Spin-column chromatography
ASJC Scopus subject areas
- Environmental Engineering
- Water Science and Technology