TY - JOUR
T1 - Development of needle-based microendoscopy for fluorescence molecular imaging of breast tumor models
AU - Chen, Chao Wei
AU - Blackwell, Tiffany R.
AU - Naphas, Renee
AU - Winnard, Paul T.
AU - Raman, Venu
AU - Glunde, Kristine
AU - Chen, Yu
N1 - Funding Information:
We thank Jerry Wierwille (University of Maryland), Nicolas Bensaid (Institut d’Optique, France), and Hongzhou Ma (Thorlabs, Inc.) for technical assistances. This work was supported in part by the Nano-Biotechnology Award of the State of Maryland, the Minta Martin Foundation, the General Research Board (GRB) Award of the University of Maryland, and the University of Maryland Baltimore (UMB) and College Park (UMCP) Seed Grant Program, and the Prevent Cancer Foundation (to Y.C.). Support from NIH P50 CA103175 (JHU ICMIC Program, to V.R.) and NIH CA134695 (to K.G.) is gratefully acknowledged.
PY - 2009/10
Y1 - 2009/10
N2 - Fluorescence molecular imaging enables the visualization of basic molecular processes such as gene expression, enzyme activity, and disease-specific molecular interactions in vivo using targeted contrast agents, and therefore, is being developed for early detection and in situ characterization of breast cancers. Recent advances in developing near-infrared fluorescent imaging contrast agents have enabled the specific labeling of human breast cancer cells in mouse model systems. In synergy with contrast agent development, this paper describes a needle-based fluorescence molecular imaging device that has the strong potential to be translated into clinical breast biopsy procedures. This microendoscopy probe is based on a gradient-index (GRIN) lens interfaced with a laser scanning microscope. Specifications of the imaging performance, including the field-of-view, transverse resolution, and focus tracking characteristics were calibrated. Orthotopic MDA-MB-231 breast cancer xenografts stably expressing the tdTomato red fluorescent protein (RFP) were used to detect the tumor cells in this tumor model as a proof of principle study. With further development, this technology, in conjunction with the development of clinically applicable, injectable fluorescent molecular imaging agents, promises to perform fluorescence molecular imaging of breast cancers in vivo for breast biopsy guidance.
AB - Fluorescence molecular imaging enables the visualization of basic molecular processes such as gene expression, enzyme activity, and disease-specific molecular interactions in vivo using targeted contrast agents, and therefore, is being developed for early detection and in situ characterization of breast cancers. Recent advances in developing near-infrared fluorescent imaging contrast agents have enabled the specific labeling of human breast cancer cells in mouse model systems. In synergy with contrast agent development, this paper describes a needle-based fluorescence molecular imaging device that has the strong potential to be translated into clinical breast biopsy procedures. This microendoscopy probe is based on a gradient-index (GRIN) lens interfaced with a laser scanning microscope. Specifications of the imaging performance, including the field-of-view, transverse resolution, and focus tracking characteristics were calibrated. Orthotopic MDA-MB-231 breast cancer xenografts stably expressing the tdTomato red fluorescent protein (RFP) were used to detect the tumor cells in this tumor model as a proof of principle study. With further development, this technology, in conjunction with the development of clinically applicable, injectable fluorescent molecular imaging agents, promises to perform fluorescence molecular imaging of breast cancers in vivo for breast biopsy guidance.
KW - Breast cancer
KW - fluorescence molecular imaging
KW - microendoscopy
KW - optical imaging
KW - tdTomato fluorescent protein
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U2 - 10.1142/S1793545809000747
DO - 10.1142/S1793545809000747
M3 - Article
AN - SCOPUS:80052077552
SN - 1793-5458
VL - 2
SP - 343
EP - 352
JO - Journal of Innovative Optical Health Sciences
JF - Journal of Innovative Optical Health Sciences
IS - 4
ER -