Development of low-cost inverted microscope to detect early growth of Mycobacterium tuberculosis in MODS culture

Mirko Zimic, Abner Velazco, Germán Comina, Jorge Coronel, Patricia Fuentes, Carmen G. Luna, Patricia Sheen, Robert H Gilman, David A J Moore

Research output: Contribution to journalArticle

Abstract

Background: The microscopic observation drug susceptibility (MODS) assay for rapid, low-cost detection of tuberculosis and multidrug resistant tuberculosis depends upon visualization of the characteristic cording colonies of Mycobacterium tuberculosis in liquid media. This has conventionally required an inverted light microscope in order to inspect the MODS culture plates from below. Few tuberculosis laboratories have this item and the capital cost of $5,000 for a high-end microscope could be a significant obstacle to MODS roll-out. Methodology: We hypothesized that the precise definition provided by costly high-specification inverted light microscopes might not be necessary for pattern recognition. Significance: In this work we describe the development of a low-cost artesenal inverted microscope that can operate in both a standard or digital mode to effectively replace the expensive commercial inverted light microscope, and an integrated system that could permit a local and remote diagnosis of tuberculosis.

Original languageEnglish (US)
Article numbere9577
JournalPLoS One
Volume5
Issue number3
DOIs
StatePublished - 2010

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Mycobacterium tuberculosis
tuberculosis
microscopes
Tuberculosis
Microscopes
light microscopes
Light
Costs and Cost Analysis
drugs
Growth
Pharmaceutical Preparations
Costs
Multidrug-Resistant Tuberculosis
Economics
Pattern recognition
Assays
Visualization
liquids
assays
Specifications

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Development of low-cost inverted microscope to detect early growth of Mycobacterium tuberculosis in MODS culture. / Zimic, Mirko; Velazco, Abner; Comina, Germán; Coronel, Jorge; Fuentes, Patricia; Luna, Carmen G.; Sheen, Patricia; Gilman, Robert H; Moore, David A J.

In: PLoS One, Vol. 5, No. 3, e9577, 2010.

Research output: Contribution to journalArticle

Zimic, Mirko ; Velazco, Abner ; Comina, Germán ; Coronel, Jorge ; Fuentes, Patricia ; Luna, Carmen G. ; Sheen, Patricia ; Gilman, Robert H ; Moore, David A J. / Development of low-cost inverted microscope to detect early growth of Mycobacterium tuberculosis in MODS culture. In: PLoS One. 2010 ; Vol. 5, No. 3.
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AU - Gilman, Robert H

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