Development of immunoglobulin G enzyme-linked immunosorbent assay for the serodiagnosis of severe acute respiratory syndrome

Pei Lan Shao, Po Ren Hsueh, Luan Yin Chang, Chun Yi Lu, Chuan Liang Kao, Yu Ping Chiang, Hsiang Yi Huang, Fu Yuan Huang, Chin Yun Lee, Lung Ji Chang, T. C. Wu, Li Min Huang

Research output: Contribution to journalArticle

Abstract

Severe acute respiratory syndrome (SARS) is an emerging infectious disease caused by a novel SARS-associated coronavirus (SARS-CoV). The clinical characteristics are high fever, rapidly progressive diffuse pneumonitis and respiratory distress. It is highly infectious through intimate contact or direct contact with infectious body fluids. Outbreaks within communities and hospitals have been reported. Development of rapid and reliable diagnostic tools is urgently needed. We developed an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), using whole virus antigen of SARS-CoV. Eighty-six serum samples collected from patients who were hospitalized for other causes were examined to determine the cut-off O.D. value. The cut-off O.D. value was defined as 0.175 by calculating the mean O.D. value of the 86 sera plus 3 standard deviations. To determine the sensitivity and specificity of the ELISA, 56 positive sera and 204 negative sera were tested. The sensitivity was 96.4% and the specificity was 100%. The results suggest that the IgG ELISA using whole virus antigen of SARS-CoV has a high sensitivity and specificity in detecting SARS IgG antibodies. This IgG ELISA is a powerful tool for serodiagnosis of SARS.

Original languageEnglish (US)
Pages (from-to)59-64
Number of pages6
JournalJournal of biomedical science
Volume12
Issue number1
DOIs
StatePublished - Jan 1 2005

Keywords

  • Coronavirus
  • Enzyme-linked immunosorbent assay
  • Serodiagnosis
  • Severe acute respiratory syndrome

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Pharmacology (medical)

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  • Cite this

    Shao, P. L., Hsueh, P. R., Chang, L. Y., Lu, C. Y., Kao, C. L., Chiang, Y. P., Huang, H. Y., Huang, F. Y., Lee, C. Y., Chang, L. J., Wu, T. C., & Huang, L. M. (2005). Development of immunoglobulin G enzyme-linked immunosorbent assay for the serodiagnosis of severe acute respiratory syndrome. Journal of biomedical science, 12(1), 59-64. https://doi.org/10.1007/s11373-004-8184-6