Abstract
Lethally irradiated rats reconstituted with syngeneic bone marrow and treated with cyclosporine (CsA) for 40 d develop a graft-vs-host disease-like syndrome (GVHD) after CsA therapy. We attempted to assess the development of auto-reactivity in these animals. Results revealed that a majority of the animals with syngeneic GVHD develop autocytotoxic T lymphocytes of the OX8 phenotype. In addition to reactivity with self, these cells were capable of lysing appropriate target cells from a variety of different rat strains. The target antigens appeared to be class II major histocompatibility antigens, because lysis could be effectively blocked by an anti-Ia monoclonal antibody. Cold target inhibition studies indicated that one effector-cell was capable of lysing various target cells, and provided evidence against a polyclonal activation of multiple anti-Ia-reactive cells. These results suggested that the anti-class II autoreactive cell-associated with syngeneic GVHD either recognizes a common class II determinant ('public ' epitope) shared by multiple strains of rats, or was polyspecific with respect to 'private' class II determinants.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 718-730 |
| Number of pages | 13 |
| Journal | Journal of Experimental Medicine |
| Volume | 161 |
| Issue number | 4 |
| State | Published - 1985 |
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ASJC Scopus subject areas
- Immunology
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Development of graft-vs-host disease-like syndrome in cyclosporine-treated rats after syngeneic bone marrow transplantation. I. Development of cytotoxic T lymphocytes with apparent polyclonal anti-Ia specificity, including autoreactivity. / Hess, A. D.; Horwitz, L.; Beschorner, W. E.; Santos, G. W.
In: Journal of Experimental Medicine, Vol. 161, No. 4, 1985, p. 718-730.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of graft-vs-host disease-like syndrome in cyclosporine-treated rats after syngeneic bone marrow transplantation. I. Development of cytotoxic T lymphocytes with apparent polyclonal anti-Ia specificity, including autoreactivity
AU - Hess, A. D.
AU - Horwitz, L.
AU - Beschorner, W. E.
AU - Santos, G. W.
PY - 1985
Y1 - 1985
N2 - Lethally irradiated rats reconstituted with syngeneic bone marrow and treated with cyclosporine (CsA) for 40 d develop a graft-vs-host disease-like syndrome (GVHD) after CsA therapy. We attempted to assess the development of auto-reactivity in these animals. Results revealed that a majority of the animals with syngeneic GVHD develop autocytotoxic T lymphocytes of the OX8 phenotype. In addition to reactivity with self, these cells were capable of lysing appropriate target cells from a variety of different rat strains. The target antigens appeared to be class II major histocompatibility antigens, because lysis could be effectively blocked by an anti-Ia monoclonal antibody. Cold target inhibition studies indicated that one effector-cell was capable of lysing various target cells, and provided evidence against a polyclonal activation of multiple anti-Ia-reactive cells. These results suggested that the anti-class II autoreactive cell-associated with syngeneic GVHD either recognizes a common class II determinant ('public ' epitope) shared by multiple strains of rats, or was polyspecific with respect to 'private' class II determinants.
AB - Lethally irradiated rats reconstituted with syngeneic bone marrow and treated with cyclosporine (CsA) for 40 d develop a graft-vs-host disease-like syndrome (GVHD) after CsA therapy. We attempted to assess the development of auto-reactivity in these animals. Results revealed that a majority of the animals with syngeneic GVHD develop autocytotoxic T lymphocytes of the OX8 phenotype. In addition to reactivity with self, these cells were capable of lysing appropriate target cells from a variety of different rat strains. The target antigens appeared to be class II major histocompatibility antigens, because lysis could be effectively blocked by an anti-Ia monoclonal antibody. Cold target inhibition studies indicated that one effector-cell was capable of lysing various target cells, and provided evidence against a polyclonal activation of multiple anti-Ia-reactive cells. These results suggested that the anti-class II autoreactive cell-associated with syngeneic GVHD either recognizes a common class II determinant ('public ' epitope) shared by multiple strains of rats, or was polyspecific with respect to 'private' class II determinants.
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M3 - Article
C2 - 2580038
AN - SCOPUS:0021878801
VL - 161
SP - 718
EP - 730
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
SN - 0022-1007
IS - 4
ER -