Development of antigen detection ELISA for the diagnosis of brugian and bancroftian filariasis using antibodies to recombinant filarial antigens Bm-SXP-1 and Wb-SXP-1

Pattabhiraman Lalitha, Devarajan Eswaran, Muniratnam Gnanasekar, Kakuturu Venkata Nagaraja Rao, Rangarajan Badri Narayanan, Alan Scott, Thomas Nutman, Perumal Kaliraj

Research output: Contribution to journalArticlepeer-review

Abstract

Antibodies specific to recombinant filarial antigens Wb-SXP-1 and Bm-SXP-1 have been used to develop a sandwich ELISA for the detection of circulating filarial antigen (CFA) in sera from patients with lymphatic filariasis caused by Wuchereria bancrofti of Brugia malayi. In patients with W. bancrofti infections, a high proportion of microfilaria (mf) positive (MF) and low proportions of patients with chronic pathology (CP) and endemic normals (EN) showed the presence of CFA. Similarly in patients with brugian infections a high proportion of mf positive individuals contained CFA while none of the patients with chronic pathology or endemic normals showed the presence of CFA. Sera from patients with other parasitic infections (OPI) like O. volvulus, Loa loa, Ascaris lumbricoides and from individuals residing in areas non-endemic to filariasis did not exhibit any reactivity. This assay shows promise for the detection of microfilaremic infections in lymphatic filariasis and its usefulness as a diagnostic tool especially in B. malayi infections, needs to be further evaluated.

Original languageEnglish (US)
Pages (from-to)327-332
Number of pages6
JournalMicrobiology and Immunology
Volume46
Issue number5
DOIs
StatePublished - Jan 1 2002

Keywords

  • Brugia malayi
  • ELISA
  • Filariasis
  • Recombinant antigen
  • Wuchereria bancrofti

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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