TY - JOUR
T1 - Development of antigen detection ELISA for the diagnosis of brugian and bancroftian filariasis using antibodies to recombinant filarial antigens Bm-SXP-1 and Wb-SXP-1
AU - Lalitha, Pattabhiraman
AU - Eswaran, Devarajan
AU - Gnanasekar, Muniratnam
AU - Rao, Kakuturu Venkata Nagaraja
AU - Narayanan, Rangarajan Badri
AU - Scott, Alan
AU - Nutman, Thomas
AU - Kaliraj, Perumal
PY - 2002/1/1
Y1 - 2002/1/1
N2 - Antibodies specific to recombinant filarial antigens Wb-SXP-1 and Bm-SXP-1 have been used to develop a sandwich ELISA for the detection of circulating filarial antigen (CFA) in sera from patients with lymphatic filariasis caused by Wuchereria bancrofti of Brugia malayi. In patients with W. bancrofti infections, a high proportion of microfilaria (mf) positive (MF) and low proportions of patients with chronic pathology (CP) and endemic normals (EN) showed the presence of CFA. Similarly in patients with brugian infections a high proportion of mf positive individuals contained CFA while none of the patients with chronic pathology or endemic normals showed the presence of CFA. Sera from patients with other parasitic infections (OPI) like O. volvulus, Loa loa, Ascaris lumbricoides and from individuals residing in areas non-endemic to filariasis did not exhibit any reactivity. This assay shows promise for the detection of microfilaremic infections in lymphatic filariasis and its usefulness as a diagnostic tool especially in B. malayi infections, needs to be further evaluated.
AB - Antibodies specific to recombinant filarial antigens Wb-SXP-1 and Bm-SXP-1 have been used to develop a sandwich ELISA for the detection of circulating filarial antigen (CFA) in sera from patients with lymphatic filariasis caused by Wuchereria bancrofti of Brugia malayi. In patients with W. bancrofti infections, a high proportion of microfilaria (mf) positive (MF) and low proportions of patients with chronic pathology (CP) and endemic normals (EN) showed the presence of CFA. Similarly in patients with brugian infections a high proportion of mf positive individuals contained CFA while none of the patients with chronic pathology or endemic normals showed the presence of CFA. Sera from patients with other parasitic infections (OPI) like O. volvulus, Loa loa, Ascaris lumbricoides and from individuals residing in areas non-endemic to filariasis did not exhibit any reactivity. This assay shows promise for the detection of microfilaremic infections in lymphatic filariasis and its usefulness as a diagnostic tool especially in B. malayi infections, needs to be further evaluated.
KW - Brugia malayi
KW - ELISA
KW - Filariasis
KW - Recombinant antigen
KW - Wuchereria bancrofti
UR - http://www.scopus.com/inward/record.url?scp=0035992633&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035992633&partnerID=8YFLogxK
U2 - 10.1111/j.1348-0421.2002.tb02703.x
DO - 10.1111/j.1348-0421.2002.tb02703.x
M3 - Article
C2 - 12139392
AN - SCOPUS:0035992633
SN - 0385-5600
VL - 46
SP - 327
EP - 332
JO - Microbiology and Immunology
JF - Microbiology and Immunology
IS - 5
ER -