Development of antibodies to HIV-1 is associated with an increase in circulating CD3+ CD4- CD8- lymphocytes

Joseph B. Margolick, Vincent Carey, Alvaro Muñoz, B. Frank Polk, Janis V. Giorgi, Kenneth D. Bauer, Richard Kaslow, Charles Rinaldo

Research output: Contribution to journalArticlepeer-review

Abstract

This study investigated whether seroconversion with respect to human immunodeficiency virus, type 1 (HIV-1) was associated with an increase in lymphocytes expressing the CD3+ CD4- CD8- phenotype. Proportions and absolute numbers of CD3+, CD4+, and CD8+ lymphocytes were determined prospectively over a 2.5-year period on 4954 homosexual and/or bisexual men participating in the Multicenter AIDS Cohort Study. Of the 4808 men whose serostatus at entry could be verified, 1745 were seropositive (SP) for antibodies to HIV-1 at entry into study, 2795 were uniformly seronegative (SN) for HIV-1 for 30 months, and 268 were seroconverters (SC) with respect to HIV-1 during this period. For each of six semiannual evaluations, proportions and numbers of CD3+ CD4- CD8- lymphocytes (calculated as CD3 - (CD4 + CD8)) were both significantly greater in the SP group than in the SN group (P < 0.001). Mean CD3+ CD4- CD8- levels in the SC group were indistinguishable from those in the SN group before seroconversion, but by 3-9 months after seroconversion the SC group demonstrated absolute numbers of CD3+ CD4- CD8- lymphocytes which were significantly increased (P < 0.001) compared to the SN group using linear regression methods with adjustment for correlation of measurements within an individual over time. An additional significant increase occurred by 21-27 months after seroconversion (P = 0.006). These results are consistent with an association of HIV-1 seroconversion with an increase in circulating T lymphocytes expressing the CD3+ CD4- CD8- phenotype (double negative T cells), a decrease in CD3- CD4- CD8+ natural killer cells, or both. An increase in double negative T cells could reflect a host defense mechanism against HIV-1 or effects of HIV-1 on T cell development.

Original languageEnglish (US)
Pages (from-to)348-361
Number of pages14
JournalClinical Immunology and Immunopathology
Volume51
Issue number3
DOIs
StatePublished - Mar 1989

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology

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