Development of an operator-independent method for seeding tissue-engineered vascular grafts

Brooks Udelsman, Narutoshi Hibino, Gustavo A. Villalona, Edward McGillicuddy, Alejandro Nieponice, Yuki Sakamoto, Shojiro Matsuda, David A. Vorp, Toshiharu Shinoka, Christopher K. Breuer

Research output: Contribution to journalArticlepeer-review

Abstract

The manual seeding of cells onto a biodegradable scaffold by pipetting is an effective method of cell seeding. However, the widespread use and ultimate clinical utility of this technique is limited by operator variability. This study was conducted to evaluate an operator-independent vacuum-seeding method for use in an upcoming clinical trial. Using bone marrow-derived mononuclear cells, we achieved seeding comparable to manually seeded scaffolds in terms of cellular attachment, distribution, and viability in vacuum-seeded grafts at vacuum pressures of -25 to -50mmHg. In conclusion, we describe an operator-independent seeding method for use in the clinical setting.

Original languageEnglish (US)
Pages (from-to)731-736
Number of pages6
JournalTissue Engineering - Part C: Methods
Volume17
Issue number7
DOIs
StatePublished - Jul 1 2011
Externally publishedYes

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Bioengineering
  • Biomedical Engineering

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