Development of an automated handheld immunoaffinity fluorometric biosensor

Micah A. Carlson, C. Brent Bargeron, Richard C. Benson, Allan B. Fraser, John D. Groopman, Harvey W. Ko, Terry E. Phillips, Paul T. Strickland, James T. Velky

Research output: Contribution to journalArticle

Abstract

A new immunoaffinity fluorometric biosensor has been developed for detecting and quantifying aflatoxins, a family of potent fungi-produced carcinogens that are commonly found in a variety of agricultural products. They have also been cited as biological agents under weapons development. The handheld, self-contained biosensor is fully automatic, highly sensitive, quick, quantitative, and requires no special storage. Concentrations from 0.1 to 50 parts per billion can be determined in less than 2 min with a 1-mL sample volume. The device operates on the principles of immunoaffinity for specificity and fluorescence for a quantitative assay. The analytic procedure is flexible so that other chemical and biological analytes can be detected with minor modifications to the current device. Advances in electro-optical components, electronics, and miniaturized fluidics were combined to produce this reliable, small, and versatile instrument.

Original languageEnglish (US)
Pages (from-to)372-377
Number of pages6
JournalJohns Hopkins APL Technical Digest (Applied Physics Laboratory)
Volume20
Issue number3
StatePublished - Dec 1 1999
Externally publishedYes

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Keywords

  • Aflatoxin
  • Automated
  • Biosensor
  • Fluorescence
  • Handheld
  • Immunoaffinity

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Carlson, M. A., Bargeron, C. B., Benson, R. C., Fraser, A. B., Groopman, J. D., Ko, H. W., Phillips, T. E., Strickland, P. T., & Velky, J. T. (1999). Development of an automated handheld immunoaffinity fluorometric biosensor. Johns Hopkins APL Technical Digest (Applied Physics Laboratory), 20(3), 372-377.