Development of an automated, handheld, immunoaffinity fluorometric biosensor

T. E. Phillips, C. B. Bargeron, R. C. Benson, M. A. Carlson, A. B. Fraser, J. D. Groopman, H. W. Ko, P. T. Strickland, J. T. Velky

Research output: Contribution to journalConference article

Abstract

A new immunoaffinity fluorometric biosensor has been developed for detecting and quantifying aflatoxins, a family of potent fungi-produced carcinogens that are commonly found in a variety of agriculture products. They have also been cited as a biological agent under weapons development. The handheld, self-contained biosensor is fully automatic, highly sensitive, quick, quantitative, and requires no special storage. Concentrations from 0.1 parts per billion (ppb) to 50 ppb can be determined in less than 2 minutes with a 1 ml sample volume. Higher concentrations (up to parts per million) can be determined by simply reducing the sample volume. The device operates on the principles of immunoaffinity for specificity and fluorescence for a quantitative assay. The analytic procedure is flexible so that other chemical and biological analytes could be detected with minor modifications to the current device.

Original languageEnglish (US)
Pages (from-to)186-192
Number of pages7
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume3913
StatePublished - Jan 1 2000
EventIn-Vitro Diagnostic Instrumentation - San Jose, CA, USA
Duration: Jan 26 2000Jan 27 2000

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Phillips, T. E., Bargeron, C. B., Benson, R. C., Carlson, M. A., Fraser, A. B., Groopman, J. D., Ko, H. W., Strickland, P. T., & Velky, J. T. (2000). Development of an automated, handheld, immunoaffinity fluorometric biosensor. Proceedings of SPIE - The International Society for Optical Engineering, 3913, 186-192.