Development of an agar-methyl cellulose clonogenic assay for cells in transitional cell carcinoma of the human bladder

R. N. Buick, T. H. Stanisic, S. E. Fry, S. E. Salmon, J. M. Trent, P. Krasovich

Research output: Contribution to journalArticle

Abstract

The authors report the development of a clonogenic assay for progenitor cells in transitional cell carcinoma of the bladder. Colony growth has been demonstrated from cells obtained both from surgical biopsies and from bladder barbotages. Electron microscopic and karyotypic evidence supports the contention that these progenitors represent a part of the population maintaining the tumor in vivo. Colony growth occurred in 9 of 11 surgical biopsy samples and in 6 of 6 bladder barbotage samples. Plating efficiency ranged up to 0.7%, and colony size was in some instances greater than 1000 cells. The assay appears potentially useful for analysis of the biology of human transitional cell carcinoma.

Original languageEnglish (US)
Pages (from-to)5051-5056
Number of pages6
JournalCancer Research
Volume39
Issue number12
StatePublished - 1979
Externally publishedYes

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Colony-Forming Units Assay
Methylcellulose
Transitional Cell Carcinoma
Agar
Urinary Bladder
Biopsy
Growth
Stem Cells
Electrons
Population
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Buick, R. N., Stanisic, T. H., Fry, S. E., Salmon, S. E., Trent, J. M., & Krasovich, P. (1979). Development of an agar-methyl cellulose clonogenic assay for cells in transitional cell carcinoma of the human bladder. Cancer Research, 39(12), 5051-5056.

Development of an agar-methyl cellulose clonogenic assay for cells in transitional cell carcinoma of the human bladder. / Buick, R. N.; Stanisic, T. H.; Fry, S. E.; Salmon, S. E.; Trent, J. M.; Krasovich, P.

In: Cancer Research, Vol. 39, No. 12, 1979, p. 5051-5056.

Research output: Contribution to journalArticle

Buick, RN, Stanisic, TH, Fry, SE, Salmon, SE, Trent, JM & Krasovich, P 1979, 'Development of an agar-methyl cellulose clonogenic assay for cells in transitional cell carcinoma of the human bladder', Cancer Research, vol. 39, no. 12, pp. 5051-5056.
Buick RN, Stanisic TH, Fry SE, Salmon SE, Trent JM, Krasovich P. Development of an agar-methyl cellulose clonogenic assay for cells in transitional cell carcinoma of the human bladder. Cancer Research. 1979;39(12):5051-5056.
Buick, R. N. ; Stanisic, T. H. ; Fry, S. E. ; Salmon, S. E. ; Trent, J. M. ; Krasovich, P. / Development of an agar-methyl cellulose clonogenic assay for cells in transitional cell carcinoma of the human bladder. In: Cancer Research. 1979 ; Vol. 39, No. 12. pp. 5051-5056.
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