Development of a simplified isotopic antiglobulin assay for the study of tumor-specific antibody

James F. Burdick; Samuel A. Wells; Alfred M. Cohen; Alfred S. Ketcham

doi:10.1016/0022-4804(73)90134-0

Development of a simplified isotopic antiglobulin assay for the study of tumor-specific antibody

James F. Burdick, Samuel A. Wells, Alfred M. Cohen, Alfred S. Ketcham

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

An adaption of the indirect isotopic antiglobulin test is described for use on a micro scale with cell monolayers. The ease of washing greatly simplifies the technique. Antibody against the tumor-specific surface antigens on a carcinogen-induced murine sarcoma is detected with high sensitivity. Antibody is detectable against the tumor in the serum of animals which have that tumor actively growing. Four of the eight MCA-induced tumors studied have a common cell-surface antigen.

Original language	English (US)
Pages (from-to)	200-206
Number of pages	7
Journal	Journal of Surgical Research
Volume	14
Issue number	3
DOIs	https://doi.org/10.1016/0022-4804(73)90134-0
State	Published - Mar 1973
Externally published	Yes

ASJC Scopus subject areas

Surgery

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10.1016/0022-4804(73)90134-0

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title = "Development of a simplified isotopic antiglobulin assay for the study of tumor-specific antibody",

abstract = "An adaption of the indirect isotopic antiglobulin test is described for use on a micro scale with cell monolayers. The ease of washing greatly simplifies the technique. Antibody against the tumor-specific surface antigens on a carcinogen-induced murine sarcoma is detected with high sensitivity. Antibody is detectable against the tumor in the serum of animals which have that tumor actively growing. Four of the eight MCA-induced tumors studied have a common cell-surface antigen.",

author = "Burdick, {James F.} and Wells, {Samuel A.} and Cohen, {Alfred M.} and Ketcham, {Alfred S.}",

note = "Funding Information: Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014. Presented at the Sixth Annual Meeting of the Association for Academic Surgery, New Orleans, November 2-4, 1972.",

year = "1973",

month = mar,

doi = "10.1016/0022-4804(73)90134-0",

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journal = "Journal of Surgical Research",

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T1 - Development of a simplified isotopic antiglobulin assay for the study of tumor-specific antibody

AU - Burdick, James F.

AU - Wells, Samuel A.

AU - Cohen, Alfred M.

AU - Ketcham, Alfred S.

N1 - Funding Information: Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014. Presented at the Sixth Annual Meeting of the Association for Academic Surgery, New Orleans, November 2-4, 1972.

PY - 1973/3

Y1 - 1973/3

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AB - An adaption of the indirect isotopic antiglobulin test is described for use on a micro scale with cell monolayers. The ease of washing greatly simplifies the technique. Antibody against the tumor-specific surface antigens on a carcinogen-induced murine sarcoma is detected with high sensitivity. Antibody is detectable against the tumor in the serum of animals which have that tumor actively growing. Four of the eight MCA-induced tumors studied have a common cell-surface antigen.

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EP - 206

JO - Journal of Surgical Research

JF - Journal of Surgical Research

IS - 3

ER -

Development of a simplified isotopic antiglobulin assay for the study of tumor-specific antibody

Abstract

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