An adaption of the indirect isotopic antiglobulin test is described for use on a micro scale with cell monolayers. The ease of washing greatly simplifies the technique. Antibody against the tumor-specific surface antigens on a carcinogen-induced murine sarcoma is detected with high sensitivity. Antibody is detectable against the tumor in the serum of animals which have that tumor actively growing. Four of the eight MCA-induced tumors studied have a common cell-surface antigen.
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