Development of a simplified isotopic antiglobulin assay for the study of tumor-specific antibody

James F. Burdick, Samuel A. Wells, Alfred M. Cohen, Alfred S. Ketcham

Research output: Contribution to journalArticle

Abstract

An adaption of the indirect isotopic antiglobulin test is described for use on a micro scale with cell monolayers. The ease of washing greatly simplifies the technique. Antibody against the tumor-specific surface antigens on a carcinogen-induced murine sarcoma is detected with high sensitivity. Antibody is detectable against the tumor in the serum of animals which have that tumor actively growing. Four of the eight MCA-induced tumors studied have a common cell-surface antigen.

Original languageEnglish (US)
Pages (from-to)200-206
Number of pages7
JournalJournal of Surgical Research
Volume14
Issue number3
DOIs
StatePublished - 1973
Externally publishedYes

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Neoplasm Antibodies
Anti-Idiotypic Antibodies
Surface Antigens
Coombs Test
Neoplasms
Sarcoma
Carcinogens
Antibodies
Serum

ASJC Scopus subject areas

  • Surgery

Cite this

Development of a simplified isotopic antiglobulin assay for the study of tumor-specific antibody. / Burdick, James F.; Wells, Samuel A.; Cohen, Alfred M.; Ketcham, Alfred S.

In: Journal of Surgical Research, Vol. 14, No. 3, 1973, p. 200-206.

Research output: Contribution to journalArticle

Burdick, James F. ; Wells, Samuel A. ; Cohen, Alfred M. ; Ketcham, Alfred S. / Development of a simplified isotopic antiglobulin assay for the study of tumor-specific antibody. In: Journal of Surgical Research. 1973 ; Vol. 14, No. 3. pp. 200-206.
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