Development of a refined tenocyte differentiation culture technique for tendon tissue engineering

Yiwei Qiu, Xiao Wang, Yaonan Zhang, Andrew J. Carr, Liwei Zhu, Zhidao Xia, Afsie Sabokbar

Research output: Contribution to journalArticle

Abstract

We have established that human tenocytes can differentiate in the absence of exogenous fetal bovine serum (FBS) but in the presence of insulin-like growth factor-1 (IGF-1) and transforming growth factor-β 3 (TGF-β 3 ). The extent of tenocyte differentiation was assessed by examining cell survival, collagen synthesis, cell morphology and expression of tenocyte differentiation markers such as scleraxis (Scx), tenomodulin (Tnmd), collagen type I (Col-I) and decorin (Dcn). Our results indicate that 50 ng/ml IGF-1 and 10 ng/ml TGF-β 3 (in the absence of FBS) were capable of maintaining in vitro human tenocyte survival in 14-day cultures. The extent of collagen synthesis and messenger ribonucleic acid expression of Scx, Tnmd, Col-I and Dcn were significantly upregulated in response to IGF-1 and TGF-β 3 . These findings have shown for the first time that human tenocytes can be maintained in long-term culture, in serum-free conditions, making this approach a suitable one for the purpose of tendon tissue engineering.

Original languageEnglish (US)
Pages (from-to)27-36
Number of pages10
JournalCells Tissues Organs
Volume197
Issue number1
DOIs
StatePublished - Dec 1 2012
Externally publishedYes

Fingerprint

Culture Techniques
Transforming Growth Factors
Somatomedins
Tissue Engineering
Tendons
Decorin
Collagen Type I
Collagen
Serum
Differentiation Antigens
Cell Survival
RNA
Survival
Tenocytes

Keywords

  • Differentiation
  • Growth factors
  • Tendon
  • Tissue engineering

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Development of a refined tenocyte differentiation culture technique for tendon tissue engineering. / Qiu, Yiwei; Wang, Xiao; Zhang, Yaonan; Carr, Andrew J.; Zhu, Liwei; Xia, Zhidao; Sabokbar, Afsie.

In: Cells Tissues Organs, Vol. 197, No. 1, 01.12.2012, p. 27-36.

Research output: Contribution to journalArticle

Qiu, Yiwei ; Wang, Xiao ; Zhang, Yaonan ; Carr, Andrew J. ; Zhu, Liwei ; Xia, Zhidao ; Sabokbar, Afsie. / Development of a refined tenocyte differentiation culture technique for tendon tissue engineering. In: Cells Tissues Organs. 2012 ; Vol. 197, No. 1. pp. 27-36.
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