Development of a novel preclinical pancreatic cancer research model: Bioluminescence image-guided focal irradiation and tumor monitoring of orthotopic xenografts

Richard Tuli, Andrew Surmak, Juvenal Reyes, Amy Hacker-Prietz, Michael Armour, Ashley Leubner, Amanda Blackford, Erik Tryggestad, Elizabeth Jaffee, John Wong, Theodore DeWeese, Joseph M. Herman

Research output: Contribution to journalArticle

Abstract

PURPOSE: We report on a novel preclinical pancreatic cancer research model that uses bioluminescence imaging (BLI)-guided irradiation of orthotopic xenograft tumors, sparing of surrounding normal tissues, and quantitative, noninvasive longitudinal assessment of treatment response. MATERIALS AND METHODS: Luciferase-expressing MiaPaCa-2 pancreatic carcinoma cells were orthotopically injected in nude mice. BLI was compared to pathologic tumor volume, and photon emission was assessed over time. BLI was correlated to positron emission tomography (PET)/computed tomography (CT) to estimate tumor dimensions. BLI and cone-beam CT (CBCT) were used to compare tumor centroid location and estimate setup error. BLI and CBCT fusion was performed to guide irradiation of tumors using the small animal radiation research platform (SARRP). DNA damage was assessed by γ-H2Ax staining. BLI was used to longitudinally monitor treatment response. RESULTS: Bioluminescence predicted tumor volume (R = 0.8984) and increased linearly as a function of time up to a 10-fold increase in tumor burden. BLI correlated with PET/CT and necropsy specimen in size (P

Original languageEnglish (US)
Pages (from-to)77-84
Number of pages8
JournalTranslational Oncology
Volume5
Issue number2
DOIs
StatePublished - Apr 2012

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Pancreatic Neoplasms
Heterografts
Tumor Burden
Cone-Beam Computed Tomography
Research
Neoplasms
Luciferases
Photons
Nude Mice
DNA Damage
Radiation
Staining and Labeling
Positron Emission Tomography Computed Tomography

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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Development of a novel preclinical pancreatic cancer research model : Bioluminescence image-guided focal irradiation and tumor monitoring of orthotopic xenografts. / Tuli, Richard; Surmak, Andrew; Reyes, Juvenal; Hacker-Prietz, Amy; Armour, Michael; Leubner, Ashley; Blackford, Amanda; Tryggestad, Erik; Jaffee, Elizabeth; Wong, John; DeWeese, Theodore; Herman, Joseph M.

In: Translational Oncology, Vol. 5, No. 2, 04.2012, p. 77-84.

Research output: Contribution to journalArticle

Tuli, Richard ; Surmak, Andrew ; Reyes, Juvenal ; Hacker-Prietz, Amy ; Armour, Michael ; Leubner, Ashley ; Blackford, Amanda ; Tryggestad, Erik ; Jaffee, Elizabeth ; Wong, John ; DeWeese, Theodore ; Herman, Joseph M. / Development of a novel preclinical pancreatic cancer research model : Bioluminescence image-guided focal irradiation and tumor monitoring of orthotopic xenografts. In: Translational Oncology. 2012 ; Vol. 5, No. 2. pp. 77-84.
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