Development of a normal human immunoglobulin G standard curve for enzyme-linked immunosorbent assay: Use for comparison of antigen efficacy

Christina M. Scheel, Sukwan Handali, Edneia C. Bueno, Azra Khan, Kathy Hancock, Armandoe Gonzalez, Hector H. Garcia, Robert H Gilman, Victor C W Tsang

Research output: Contribution to journalArticle

Abstract

Internal standard reference curves are used in enzyme-linked immunosorbent assay (ELISA) plates to control for inter- and intra-assay variance. To compare the diagnostic potential of multiple T. solium antigens on an unbiased, universal scale, we have created a standard curve using normal, human immunoglobulin G (hIgG). The hIgG curve is inexpensive and simple to prepare, and remains stable at 5°C for at least one year, with a coefficient of variance of less than 10%. The hIgG standard curve has proven a critical tool for the comparison of several diagnostic antigens slated for assay development.

Original languageEnglish (US)
Pages (from-to)173-181
Number of pages9
JournalJournal of Immunoassay and Immunochemistry
Volume27
Issue number2
DOIs
StatePublished - Jul 1 2006

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Immunosorbents
Assays
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Antigens
Enzymes
Viral Tumor Antigens

Keywords

  • Antigen efficacy
  • Assay development
  • Diagnosis
  • ELISA
  • Immunoglobulin G
  • Standard curve

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

Development of a normal human immunoglobulin G standard curve for enzyme-linked immunosorbent assay : Use for comparison of antigen efficacy. / Scheel, Christina M.; Handali, Sukwan; Bueno, Edneia C.; Khan, Azra; Hancock, Kathy; Gonzalez, Armandoe; Garcia, Hector H.; Gilman, Robert H; Tsang, Victor C W.

In: Journal of Immunoassay and Immunochemistry, Vol. 27, No. 2, 01.07.2006, p. 173-181.

Research output: Contribution to journalArticle

Scheel, Christina M. ; Handali, Sukwan ; Bueno, Edneia C. ; Khan, Azra ; Hancock, Kathy ; Gonzalez, Armandoe ; Garcia, Hector H. ; Gilman, Robert H ; Tsang, Victor C W. / Development of a normal human immunoglobulin G standard curve for enzyme-linked immunosorbent assay : Use for comparison of antigen efficacy. In: Journal of Immunoassay and Immunochemistry. 2006 ; Vol. 27, No. 2. pp. 173-181.
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