Development of a non-invasive optical imaging method for tracking vascular gene expression

H. H. Chen, A. Kumar, Y. Yang, D. Wang, D. Maouyo, N. M. Fried, X. Yang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Gene therapy is an exciting frontier in modern medicine. To date, no imaging modalities are available for monitoring vascular gene therapy. Green fluorescent protein (GFP) has become an increasingly common marker for gene therapy. We have developed an optical imaging method to track vascular gene expression by detecting fluorescence emitted from GFP or red fluorescent protein (RFP) in arterial walls following gene transfer. We surgically transferred GFP- and RFP-vectors into the femoral and carotid arteries of three New Zealand white rabbits. Excitation light was transmitted through a fiber-optic ring-light (Nevoscope) and GFP and RFP fluorescence was detected by a charge coupled device (CCD) camera. Direct contact images of the target arteries demonstrated that this method was capable of both discriminating between normal and transferred arterial tissues and mapping fluorescent protein localization. Subsequent measurements by confocal microscopy showed statistically significant differences in average fluorescent signal intensity between the control and transferred tissues. This result was corroborated by immunohistochemical staining. These preliminary results are encouraging evidence that the optical imaging method can be developed further to be performed non-invasively and in vivo in a clinical setting.

Original languageEnglish (US)
Title of host publicationAnnual Reports of the Research Reactor Institute, Kyoto University
Pages2840-2843
Number of pages4
Volume3
Publication statusPublished - 2001
Event23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society - Istanbul, Turkey
Duration: Oct 25 2001Oct 28 2001

Other

Other23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society
CountryTurkey
CityIstanbul
Period10/25/0110/28/01

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Keywords

  • Cardiovascular disease
  • Confocal microscopy
  • Gene therapy
  • Green fluorescent protein (GFP)
  • Optical imaging

ASJC Scopus subject areas

  • Energy Engineering and Power Technology
  • Mechanical Engineering

Cite this

Chen, H. H., Kumar, A., Yang, Y., Wang, D., Maouyo, D., Fried, N. M., & Yang, X. (2001). Development of a non-invasive optical imaging method for tracking vascular gene expression. In Annual Reports of the Research Reactor Institute, Kyoto University (Vol. 3, pp. 2840-2843)