TY - JOUR
T1 - Development of a new orthotopic animal model of metastatic liver cancer in the rabbit VX2 model
T2 - Effect on metastases after partial hepatectomy, intra-arterial treatment with 3-bromopyruvate and chemoembolization
AU - Vossen, Josephina A.
AU - Buijs, Manon
AU - Syed, Labiq
AU - Kutiyanwala, Fatima
AU - Kutiyanwala, Mustafa
AU - Geschwind, Jean Francois H.
AU - Vali, Mustafa
N1 - Funding Information:
Acknowledgments The work was supported by NCI RO1 CA100883-01, the Abdulrahman Abdulmalik Research Fund and the Charles Wallace Pratt Research Fund
PY - 2008/11
Y1 - 2008/11
N2 - Aim: The aim of our study was to compare the influence of partial hepatectomy, intra-arterial treatment with 3-BrPA and TACE on regional and distant metastases. In order to achieve our objective, we tested the feasibility of both resection, intra-arterial therapy with 3-BrPA and TACE of VX2 liver cancer in New Zealand White rabbits. Methods: VX2 tumors were implanted in the left lateral lobe of the liver of 20 rabbits. Tumors were allowed to grow for 14 days. Rabbits were divided in four groups. Group 1 (n = 2) was sacrificed 14 days post implantation. Group 2, 3 and 4 (n = 6) underwent left lateral hepatectomy, a 1 h intra-arterial infusion with 3-BrPA and TACE respectively. Animals in each group were further subdivided into three groups of two animals each corresponding to the time-point of sacrifice after the procedure (7, 14 and 21 days respectively). After sacrifice, organs were harvested, fixed and analyzed. Results: Pathologic examination showed lung metastases in all 20 rabbits. Abdominal cavity dissemination was seen in five rabbits in Group 2, two rabbits in Group 3 and all rabbits in Group 4. Kidney metastases were seen in two rabbits treated with TACE. Conclusion: The VX2 rabbit model of liver cancer is a suitable model to compare the influence of partial hepatectomy, intra-arterial treatment with 3-BrPA and TACE on tumor recurrence in the form of regional and distant metastases. Our results indicate that intra-arterial delivery of 3-BrPA may result in a favorable metastatic profile when compared to both liver resection and TACE.
AB - Aim: The aim of our study was to compare the influence of partial hepatectomy, intra-arterial treatment with 3-BrPA and TACE on regional and distant metastases. In order to achieve our objective, we tested the feasibility of both resection, intra-arterial therapy with 3-BrPA and TACE of VX2 liver cancer in New Zealand White rabbits. Methods: VX2 tumors were implanted in the left lateral lobe of the liver of 20 rabbits. Tumors were allowed to grow for 14 days. Rabbits were divided in four groups. Group 1 (n = 2) was sacrificed 14 days post implantation. Group 2, 3 and 4 (n = 6) underwent left lateral hepatectomy, a 1 h intra-arterial infusion with 3-BrPA and TACE respectively. Animals in each group were further subdivided into three groups of two animals each corresponding to the time-point of sacrifice after the procedure (7, 14 and 21 days respectively). After sacrifice, organs were harvested, fixed and analyzed. Results: Pathologic examination showed lung metastases in all 20 rabbits. Abdominal cavity dissemination was seen in five rabbits in Group 2, two rabbits in Group 3 and all rabbits in Group 4. Kidney metastases were seen in two rabbits treated with TACE. Conclusion: The VX2 rabbit model of liver cancer is a suitable model to compare the influence of partial hepatectomy, intra-arterial treatment with 3-BrPA and TACE on tumor recurrence in the form of regional and distant metastases. Our results indicate that intra-arterial delivery of 3-BrPA may result in a favorable metastatic profile when compared to both liver resection and TACE.
KW - 3-Bromopyruvate
KW - Animal model
KW - Cancer
KW - Chemoembolization
KW - HCC
KW - Hepatectomy
KW - Metastases
KW - Transarterial
KW - VX2
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U2 - 10.1007/s10585-008-9195-x
DO - 10.1007/s10585-008-9195-x
M3 - Article
C2 - 18649116
AN - SCOPUS:53949087157
SN - 0262-0898
VL - 25
SP - 811
EP - 817
JO - Clinical and Experimental Metastasis
JF - Clinical and Experimental Metastasis
IS - 7
ER -