Development of a multicompanent candidate vaccine for HIV-1

Jong J. Kim, Velpandi Ayyavoo, Mark L. Bagarazzi, Michael Chattergoon, Jean D. Boyer, Bin Wang, David B. Weiner

Research output: Contribution to journalArticle

Abstract

Nucleic acid or DNA immunization represents a novel approach to both vaccine and immune therapeutic development. DNA vaccination induces antigen- specific cellular and humoral immune responses through the delivery of non- replicating transcription units which drive the synthesis of specific foreign proteins within the inoculated host. We have previously reported on the potential use of DNA immunization as a novel vaccine strategy for HIV-1. We found that both antigen-specific cellular and humoral immune responses could be induced in viva with various DNA vaccine constructs against different antigenic targets within HIV-1. In order to enhance the DNA vaccine's ability to elicit cell-mediated immune responses, we co-delivered plasmids encoding costimulatory molecule B7 and interleukin-12 genes with DNA vaccine for HIV- 1. We observed a dramatic increase in both antigen-specific T helper cell proliferation and CTL response. Eventual development of successful vaccines for HIV-1 would likely involve targeting multiple antigenic components of the virus to direct and empower the immune system to protect the host from viral infection. We present here the utility of multicomponent DNA immunization to elicit specific humoral and cell-mediated immune responses against different antigenic targets of HIV-1 as well as the ability of this immunization strategy to achieve significant enhancements of antigen-specific cellular immune responses.

Original languageEnglish (US)
Pages (from-to)879-883
Number of pages5
JournalVaccine
Volume15
Issue number8
DOIs
StatePublished - Jun 1 1997
Externally publishedYes

Keywords

  • B7
  • DNA Vaccines
  • HIV-1
  • IL-12
  • Immune responses

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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  • Cite this

    Kim, J. J., Ayyavoo, V., Bagarazzi, M. L., Chattergoon, M., Boyer, J. D., Wang, B., & Weiner, D. B. (1997). Development of a multicompanent candidate vaccine for HIV-1. Vaccine, 15(8), 879-883. https://doi.org/10.1016/S0264-410X(96)00260-5