Development of a multiantigen panel for improved detection of borrelia burgdorferi infection in early lyme disease

Lauren J. Lahey, Michael W. Panas, Rong Mao, Michelle Delanoy, John J. Flanagan, Steven R. Binder, Alison W. Rebman, Jose G. Montoya, Mark J. Soloski, Allen C. Steere, Raymond J. Dattwyler, Paul M. Arnaboldi, John N. Aucott, William H. Robinson

Research output: Research - peer-reviewArticle

Abstract

The current standard for laboratory diagnosis of Lyme disease in the United States is serologic detection of antibodies against Borrelia burgdorferi. The Centers for Disease Control and Prevention recommends a two-tiered testing algorithm; however, this scheme has limited sensitivity for detecting early Lyme disease. Thus, there is a need to improve diagnostics for Lyme disease at the early stage, when antibiotic treatment is highly efficacious. We examined novel and established antigen markers to develop a multiplex panel that identifies early infection using the combined sensitivity of multiple markers while simultaneously maintaining high specificity by requiring positive results for two markers to designate a positive test. Ten markers were selected from our initial analysis of 62 B. burgdorferi surface proteins and synthetic peptides by assessing binding of IgG and IgM to each in a training set of Lyme disease patient samples and controls. In a validation set, this 10-antigen panel identified a higher proportion of early-Lyme-disease patients as positive at the baseline or posttreatment visit than two-tiered testing (87.5% and 67.5%, respectively; P

LanguageEnglish (US)
Pages3834-3841
Number of pages8
JournalJournal of Clinical Microbiology
Volume53
Issue number12
DOIs
StatePublished - Dec 1 2015

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Borrelia Infections
Borrelia burgdorferi
Lyme Disease
Clinical Laboratory Techniques
Differentiation Antigens
Centers for Disease Control and Prevention (U.S.)
Immunoglobulin M
Membrane Proteins
Immunoglobulin G
Anti-Bacterial Agents
Antigens
Peptides
Antibodies
Infection
Therapeutics

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Lahey, L. J., Panas, M. W., Mao, R., Delanoy, M., Flanagan, J. J., Binder, S. R., ... Robinson, W. H. (2015). Development of a multiantigen panel for improved detection of borrelia burgdorferi infection in early lyme disease. Journal of Clinical Microbiology, 53(12), 3834-3841. DOI: 10.1128/JCM.02111-15

Development of a multiantigen panel for improved detection of borrelia burgdorferi infection in early lyme disease. / Lahey, Lauren J.; Panas, Michael W.; Mao, Rong; Delanoy, Michelle; Flanagan, John J.; Binder, Steven R.; Rebman, Alison W.; Montoya, Jose G.; Soloski, Mark J.; Steere, Allen C.; Dattwyler, Raymond J.; Arnaboldi, Paul M.; Aucott, John N.; Robinson, William H.

In: Journal of Clinical Microbiology, Vol. 53, No. 12, 01.12.2015, p. 3834-3841.

Research output: Research - peer-reviewArticle

Lahey, LJ, Panas, MW, Mao, R, Delanoy, M, Flanagan, JJ, Binder, SR, Rebman, AW, Montoya, JG, Soloski, MJ, Steere, AC, Dattwyler, RJ, Arnaboldi, PM, Aucott, JN & Robinson, WH 2015, 'Development of a multiantigen panel for improved detection of borrelia burgdorferi infection in early lyme disease' Journal of Clinical Microbiology, vol 53, no. 12, pp. 3834-3841. DOI: 10.1128/JCM.02111-15
Lahey LJ, Panas MW, Mao R, Delanoy M, Flanagan JJ, Binder SR et al. Development of a multiantigen panel for improved detection of borrelia burgdorferi infection in early lyme disease. Journal of Clinical Microbiology. 2015 Dec 1;53(12):3834-3841. Available from, DOI: 10.1128/JCM.02111-15
Lahey, Lauren J. ; Panas, Michael W. ; Mao, Rong ; Delanoy, Michelle ; Flanagan, John J. ; Binder, Steven R. ; Rebman, Alison W. ; Montoya, Jose G. ; Soloski, Mark J. ; Steere, Allen C. ; Dattwyler, Raymond J. ; Arnaboldi, Paul M. ; Aucott, John N. ; Robinson, William H./ Development of a multiantigen panel for improved detection of borrelia burgdorferi infection in early lyme disease. In: Journal of Clinical Microbiology. 2015 ; Vol. 53, No. 12. pp. 3834-3841
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