Development of a Mouse Pain Scale Using Sub-second Behavioral Mapping and Statistical Modeling

Ishmail Abdus-Saboor, Nathan T. Fried, Mark Lay, Justin Burdge, Kathryn Swanson, R. Fischer, Jessica Jones, P. Dong, Weihua Cai, Xinying Guo, Yuan Xiang Tao, John Bethea, Minghong Ma, Xinzhong Dong, Long Ding, Wenqin Luo

Research output: Contribution to journalArticle

Abstract

Rodents are the main model systems for pain research, but determining their pain state is challenging. To develop an objective method to assess pain sensation in mice, we adopt high-speed videography to capture sub-second behavioral features following hind paw stimulation with both noxious and innocuous stimuli and identify several differentiating parameters indicating the affective and reflexive aspects of nociception. Using statistical modeling and machine learning, we integrate these parameters into a single index and create a “mouse pain scale,” which allows us to assess pain sensation in a graded manner for each withdrawal. We demonstrate the utility of this method by determining sensations triggered by three different von Frey hairs and optogenetic activation of two different nociceptor populations. Our behavior-based “pain scale” approach will help improve the rigor and reproducibility of using withdrawal reflex assays to assess pain sensation in mice. Abdus-Saboor et al. develop a behavior-centered “mouse pain scale” using high-speed videography, statistical modeling, and machine learning. With this method, they assess the sensation induced by noxious, innocuous, and optogenetic stimuli. This method will improve the reliability of using the mouse hind paw withdrawal to measure pain.

Original languageEnglish (US)
Pages (from-to)1623-1634.e4
JournalCell Reports
Volume28
Issue number6
DOIs
StatePublished - Aug 6 2019

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Keywords

  • high-speed imaging
  • machine learning
  • mouse pain behavior
  • nociceptors
  • optogenetics
  • pain scale
  • principle component analysis
  • somatosensation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Abdus-Saboor, I., Fried, N. T., Lay, M., Burdge, J., Swanson, K., Fischer, R., Jones, J., Dong, P., Cai, W., Guo, X., Tao, Y. X., Bethea, J., Ma, M., Dong, X., Ding, L., & Luo, W. (2019). Development of a Mouse Pain Scale Using Sub-second Behavioral Mapping and Statistical Modeling. Cell Reports, 28(6), 1623-1634.e4. https://doi.org/10.1016/j.celrep.2019.07.017