Development of a microwave - Accelerated metal-enhanced fluorescence 40 second, <100 cfu/mL point of care assay for the detection of Chlamydia trachomatis

Yongxia Zhang; Patricia Agreda; Shannon Kelley; Charlotte Gaydos; Chris D. Geddes

doi:10.1109/TBME.2010.2066275

Development of a microwave - Accelerated metal-enhanced fluorescence 40 second, <100 cfu/mL point of care assay for the detection of Chlamydia trachomatis

Yongxia Zhang, Patricia Agreda, Shannon Kelley, Charlotte Gaydos, Chris D. Geddes

School of Medicine

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

An inexpensive technology to both lyse Chlamydia trachomatis (CT) and detect DNA released from CT within 40 s is demonstrated. In a microwave cavity, energy is highly focused using 100-nm gold films with "bow-tie" structures to lyse CT within 10 s. The ultrafast detection of the released DNA from less than 100 cfu/mL CT is accomplished in an additional 30 s by employing the microwave-accelerated metal-enhanced fluorescence technique. This new "release and detect" platform technology is a highly attractive alternative method for the lysing of bacteria, DNA extraction, and the fast quantification of bacteria and potentially other pathogenic species and cells as well. Our approach is a significant step forward for the development of a point of care test for CT.

Original language	English (US)
Pages (from-to)	781-784
Number of pages	4
Journal	IEEE Transactions on Biomedical Engineering
Volume	58
Issue number	3 PART 2
DOIs	https://doi.org/10.1109/TBME.2010.2066275
State	Published - Mar 2011

Keywords

Chlamydia trachomatis (CT)
lysis
microwave-accelerated metal-enhanced fluorescence (MAMEF)

ASJC Scopus subject areas

Biomedical Engineering

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10.1109/TBME.2010.2066275

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Development of a microwave - Accelerated metal-enhanced fluorescence 40 second, <100 cfu/mL point of care assay for the detection of Chlamydia trachomatis. / Zhang, Yongxia; Agreda, Patricia; Kelley, Shannon et al.
In: IEEE Transactions on Biomedical Engineering, Vol. 58, No. 3 PART 2, 03.2011, p. 781-784.

Research output: Contribution to journal › Article › peer-review

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abstract = "An inexpensive technology to both lyse Chlamydia trachomatis (CT) and detect DNA released from CT within 40 s is demonstrated. In a microwave cavity, energy is highly focused using 100-nm gold films with {"}bow-tie{"} structures to lyse CT within 10 s. The ultrafast detection of the released DNA from less than 100 cfu/mL CT is accomplished in an additional 30 s by employing the microwave-accelerated metal-enhanced fluorescence technique. This new {"}release and detect{"} platform technology is a highly attractive alternative method for the lysing of bacteria, DNA extraction, and the fast quantification of bacteria and potentially other pathogenic species and cells as well. Our approach is a significant step forward for the development of a point of care test for CT.",

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author = "Yongxia Zhang and Patricia Agreda and Shannon Kelley and Charlotte Gaydos and Geddes, {Chris D.}",

note = "Funding Information: Manuscript received July 22, 2010; accepted August 2, 2010. Date of publication August 12, 2010; date of current version February 18, 2011. This work was supported by the National Institute of Biomedical Imaging and Bioengineering, NIH, under Grant 1U54 EB007958-4. Asterisk indicates corresponding author.",

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AU - Gaydos, Charlotte

AU - Geddes, Chris D.

N1 - Funding Information: Manuscript received July 22, 2010; accepted August 2, 2010. Date of publication August 12, 2010; date of current version February 18, 2011. This work was supported by the National Institute of Biomedical Imaging and Bioengineering, NIH, under Grant 1U54 EB007958-4. Asterisk indicates corresponding author.

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Development of a microwave - Accelerated metal-enhanced fluorescence 40 second, <100 cfu/mL point of care assay for the detection of Chlamydia trachomatis

Abstract

Keywords

ASJC Scopus subject areas

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