Development of a microwave - Accelerated metal-enhanced fluorescence 40 second, <100 cfu/mL point of care assay for the detection of Chlamydia trachomatis

Yongxia Zhang, Patricia Agreda, Shannon Kelley, Charlotte Gaydos, Chris D. Geddes

Research output: Contribution to journalArticlepeer-review

Abstract

An inexpensive technology to both lyse Chlamydia trachomatis (CT) and detect DNA released from CT within 40 s is demonstrated. In a microwave cavity, energy is highly focused using 100-nm gold films with "bow-tie" structures to lyse CT within 10 s. The ultrafast detection of the released DNA from less than 100 cfu/mL CT is accomplished in an additional 30 s by employing the microwave-accelerated metal-enhanced fluorescence technique. This new "release and detect" platform technology is a highly attractive alternative method for the lysing of bacteria, DNA extraction, and the fast quantification of bacteria and potentially other pathogenic species and cells as well. Our approach is a significant step forward for the development of a point of care test for CT.

Original languageEnglish (US)
Pages (from-to)781-784
Number of pages4
JournalIEEE Transactions on Biomedical Engineering
Volume58
Issue number3 PART 2
DOIs
StatePublished - Mar 2011

Keywords

  • Chlamydia trachomatis (CT)
  • lysis
  • microwave-accelerated metal-enhanced fluorescence (MAMEF)

ASJC Scopus subject areas

  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Development of a microwave - Accelerated metal-enhanced fluorescence 40 second, <100 cfu/mL point of care assay for the detection of Chlamydia trachomatis'. Together they form a unique fingerprint.

Cite this