Development of a microwave - Accelerated metal-enhanced fluorescence 40 second,

Yongxia Zhang, Patricia Agreda, Shannon Kelley, Charlotte A Gaydos, Chris D. Geddes

Research output: Contribution to journalArticle

Abstract

An inexpensive technology to both lyse Chlamydia trachomatis (CT) and detect DNA released from CT within 40 s is demonstrated. In a microwave cavity, energy is highly focused using 100-nm gold films with "bow-tie" structures to lyse CT within 10 s. The ultrafast detection of the released DNA from less than 100 cfu/mL CT is accomplished in an additional 30 s by employing the microwave-accelerated metal-enhanced fluorescence technique. This new "release and detect" platform technology is a highly attractive alternative method for the lysing of bacteria, DNA extraction, and the fast quantification of bacteria and potentially other pathogenic species and cells as well. Our approach is a significant step forward for the development of a point of care test for CT.

Original languageEnglish (US)
Pages (from-to)781-784
Number of pages4
JournalIEEE Transactions on Biomedical Engineering
Volume58
Issue number3 PART 2
DOIs
StatePublished - Mar 2011

Fingerprint

DNA
Fluorescence
Microwaves
Bacteria
Metals
Gold

Keywords

  • Chlamydia trachomatis (CT)
  • lysis
  • microwave-accelerated metal-enhanced fluorescence (MAMEF)

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Development of a microwave - Accelerated metal-enhanced fluorescence 40 second, . / Zhang, Yongxia; Agreda, Patricia; Kelley, Shannon; Gaydos, Charlotte A; Geddes, Chris D.

In: IEEE Transactions on Biomedical Engineering, Vol. 58, No. 3 PART 2, 03.2011, p. 781-784.

Research output: Contribution to journalArticle

Zhang, Yongxia ; Agreda, Patricia ; Kelley, Shannon ; Gaydos, Charlotte A ; Geddes, Chris D. / Development of a microwave - Accelerated metal-enhanced fluorescence 40 second, . In: IEEE Transactions on Biomedical Engineering. 2011 ; Vol. 58, No. 3 PART 2. pp. 781-784.
@article{96e6645e0c0744c9b186580681b67a70,
title = "Development of a microwave - Accelerated metal-enhanced fluorescence 40 second,",
abstract = "An inexpensive technology to both lyse Chlamydia trachomatis (CT) and detect DNA released from CT within 40 s is demonstrated. In a microwave cavity, energy is highly focused using 100-nm gold films with {"}bow-tie{"} structures to lyse CT within 10 s. The ultrafast detection of the released DNA from less than 100 cfu/mL CT is accomplished in an additional 30 s by employing the microwave-accelerated metal-enhanced fluorescence technique. This new {"}release and detect{"} platform technology is a highly attractive alternative method for the lysing of bacteria, DNA extraction, and the fast quantification of bacteria and potentially other pathogenic species and cells as well. Our approach is a significant step forward for the development of a point of care test for CT.",
keywords = "Chlamydia trachomatis (CT), lysis, microwave-accelerated metal-enhanced fluorescence (MAMEF)",
author = "Yongxia Zhang and Patricia Agreda and Shannon Kelley and Gaydos, {Charlotte A} and Geddes, {Chris D.}",
year = "2011",
month = "3",
doi = "10.1109/TBME.2010.2066275",
language = "English (US)",
volume = "58",
pages = "781--784",
journal = "IEEE Transactions on Biomedical Engineering",
issn = "0018-9294",
publisher = "IEEE Computer Society",
number = "3 PART 2",

}

TY - JOUR

T1 - Development of a microwave - Accelerated metal-enhanced fluorescence 40 second,

AU - Zhang, Yongxia

AU - Agreda, Patricia

AU - Kelley, Shannon

AU - Gaydos, Charlotte A

AU - Geddes, Chris D.

PY - 2011/3

Y1 - 2011/3

N2 - An inexpensive technology to both lyse Chlamydia trachomatis (CT) and detect DNA released from CT within 40 s is demonstrated. In a microwave cavity, energy is highly focused using 100-nm gold films with "bow-tie" structures to lyse CT within 10 s. The ultrafast detection of the released DNA from less than 100 cfu/mL CT is accomplished in an additional 30 s by employing the microwave-accelerated metal-enhanced fluorescence technique. This new "release and detect" platform technology is a highly attractive alternative method for the lysing of bacteria, DNA extraction, and the fast quantification of bacteria and potentially other pathogenic species and cells as well. Our approach is a significant step forward for the development of a point of care test for CT.

AB - An inexpensive technology to both lyse Chlamydia trachomatis (CT) and detect DNA released from CT within 40 s is demonstrated. In a microwave cavity, energy is highly focused using 100-nm gold films with "bow-tie" structures to lyse CT within 10 s. The ultrafast detection of the released DNA from less than 100 cfu/mL CT is accomplished in an additional 30 s by employing the microwave-accelerated metal-enhanced fluorescence technique. This new "release and detect" platform technology is a highly attractive alternative method for the lysing of bacteria, DNA extraction, and the fast quantification of bacteria and potentially other pathogenic species and cells as well. Our approach is a significant step forward for the development of a point of care test for CT.

KW - Chlamydia trachomatis (CT)

KW - lysis

KW - microwave-accelerated metal-enhanced fluorescence (MAMEF)

UR - http://www.scopus.com/inward/record.url?scp=79952028863&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952028863&partnerID=8YFLogxK

U2 - 10.1109/TBME.2010.2066275

DO - 10.1109/TBME.2010.2066275

M3 - Article

C2 - 20709634

AN - SCOPUS:79952028863

VL - 58

SP - 781

EP - 784

JO - IEEE Transactions on Biomedical Engineering

JF - IEEE Transactions on Biomedical Engineering

SN - 0018-9294

IS - 3 PART 2

ER -