Development of a high-performance liquid chromatographic method to determine the concentration of karenitecin, a novel highly lipophilic camptothecin derivative, in human plasma and urine

Judith A. Smith, Fredrick Hausheer, Robert A. Newman, Timothy L. Madden

Research output: Contribution to journalArticle

Abstract

Karenitecin is a novel, highly lipophilic camptothecin derivative with potent anticancer potential. We have developed a sensitive high-performance liquid chromatographic method for the determination of karenitecin concentration in human plasma and urine. Karenitecin was isolated from human plasma and urine using solid-phase extraction. Separation was achieved by gradient elution, using a water and acetonitrile mobile phase, on an ODS analytical column. Karenitecin was detected using fluorescence detection at excitation and emission wavelengths of 370 and 490 nm, respectively. Retention time for karenitecin was 16.2±0.5 min and 8.0±0.2 min for camptothecin, the internal standard. The karenitecin peak was baseline resolved, with the nearest peak at 3.1 min distance. Using normal volunteer plasma and urine from multiple individuals, as well as samples from the 50 patients analyzed to date, no interfering peaks were detected. Inter- and intra-day coefficients of variance were

Original languageEnglish (US)
Pages (from-to)117-124
Number of pages8
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume759
Issue number1
DOIs
StatePublished - Aug 5 2001
Externally publishedYes

Keywords

  • Camptothecin
  • Karenitecin

ASJC Scopus subject areas

  • Chemistry(all)

Fingerprint Dive into the research topics of 'Development of a high-performance liquid chromatographic method to determine the concentration of karenitecin, a novel highly lipophilic camptothecin derivative, in human plasma and urine'. Together they form a unique fingerprint.

  • Cite this