Development and validation of high-throughput liquid chromatography-tandem mass spectrometric method for simultaneous quantification of loratadine and desloratadine in human plasma

G. Srinubabu, Rajaram S. Patel, Vinay P. Shedbalkar, Allam Appa Rao, M. Narasimha Rao, Veera Venkata Ratnam Bandaru

Research output: Contribution to journalArticle

Abstract

As a continuation of effort to improve our high flow on-line bioanalytical approach for high-throughput quantification of drugs and metabolites in plasma by high-throughput liquid chromatography tandem mass spectrometry (HTLC-MS/MS), we have developed a simple, sensitive and reliable method for simultaneous quantification of loratadine and desloratadine in human plasma. We have performed on-line coupling of extraction with Cyclone P 50 mm × 0.5 mm 50 μm HTLC column and chromatographic separation is performed with Zorbax XDB C18 50 mm × 2.1 mm 5 μm, followed by quantification with mass detector. The method is validated and showed good performances in terms of linearity, sensitivity, precision, accuracy and stability. A marked improvement in sample throughput efficiency is realized with this method and the proposed method will be useful for pharmacokinetic and/or bioequivalence studies.

Original languageEnglish (US)
Pages (from-to)202-208
Number of pages7
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume860
Issue number2
DOIs
StatePublished - Dec 15 2007

Keywords

  • Desloratadine
  • High-throughput liquid chromatography
  • Human plasma
  • Loratadine
  • Mass spectrometric detection
  • Validation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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