Development and evaluation of porcine cysticercosis quickelisa in triturus® EIA analyzer

Sukwan Handali, Sowmya Pattabhi, Yeuk Mui Lee, Maria Silva-Ibanez, Victor A. Kovalenko, Andrew E. Levin, Armando E. Gonzalez, Jacquelin M. Roberts, Hector H. Garcia, Robert H. Gilman, Kathy Hancock, Victor C.W. Tsang

Research output: Contribution to journalArticlepeer-review

Abstract

We evaluated three diagnostic antigens (recombinant GP50, recombinant T24H, and synthetic Ts18var1) for cysticercosis and found that all three performed well in detecting cysticercosis in humans and pigs in several assay formats. These antigens were adapted to a new antibody detection format (QuickELISA). With one single incubation step which involves all reactants except the enzyme substrate, the QuickELISA is particularly suited for automation. We formatted the QuickELISA for the Triturus EIA analyzer for testing large numbers of samples. We found that in QuickELISA formats rGP50 and rT24H have better sensitivity and specificity than sTs18var1 for detecting porcine cysticercosis.

Original languageEnglish (US)
Pages (from-to)60-70
Number of pages11
JournalJournal of Immunoassay and Immunochemistry
Volume31
Issue number1
DOIs
StatePublished - Jan 1 2010

Keywords

  • Assay development
  • Cysticercosis
  • ELISA
  • Pig
  • Serological diagnosis
  • Taenia solium

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Medical Laboratory Technology

Fingerprint Dive into the research topics of 'Development and evaluation of porcine cysticercosis quickelisa <sup>™</sup> in triturus<sup>®</sup> EIA analyzer'. Together they form a unique fingerprint.

Cite this