Detoxification of Lignocellulose Hydrolysates

Biochemical and Metabolic Engineering Toward White Biotechnology

Anuj K. Chandel, Silvio Silvério da Silva, Om V. Singh

Research output: Contribution to journalArticle

Abstract

Chemical hydrolysis of lignocellulosic biomass (LB) produces a number of inhibitors in addition to sugars. These inhibitors include lignin-derived phenolics, carbohydrate-derived furans, and weak acids that have shown a marked effect on the productivities of various metabolites and the growth of biocatalysts in the fermentative reaction. In the past, a number of physicochemical and biological approaches have been proposed to overcome these fermentation inhibitors, including modified fermentative strategies. Additionally, the timely intervention of genetic engineering has provided an impetus to develop suitable technologies for the detoxification of lignocellulosics in biorefineries. However, the improvements in detoxification strategies for lignocellulose hydrolysates have resulted in significant loss of sugars after detoxification. Hydrolysis of myco-LB (LB after fungal pretreatment) has been recognized as a promising approach to avoid fermentation inhibitors and improve total sugar recovery. Biotechnological inventions have also made it possible to widen the range of suitable biocatalysts for biorefineries by microbial-routed induction of enzymatic expression for the elimination of inhibitors, eventually improving ethanol production from acid hydrolysates. This article aims to highlight the strategies that have been adopted to detoxify lignocellulosic hydrolysates and their effects on the chemical composition of the hydrolysates to improve the fermentability of lignocellulosics. In addition, genetic manipulation could widen the availability and variety of substrates and modify the metabolic routes to produce bioethanol or other value-added compounds in an efficient manner.

Original languageEnglish (US)
Pages (from-to)388-401
Number of pages14
JournalBioenergy Research
Volume6
Issue number1
DOIs
StatePublished - 2013
Externally publishedYes

Fingerprint

Biochemical engineering
Metabolic engineering
Detoxification
lignocellulose
metabolic engineering
Biotechnology
hydrolysates
Sugars
biotechnology
Biomass
Biocatalysts
biorefining
Fermentation
Hydrolysis
sugars
genetic engineering
Genetic engineering
biomass
Bioethanol
Acids

Keywords

  • Bioethanol
  • d-Xylitol
  • Detoxification
  • Fermentation
  • Lignocellulose hydrolysis
  • Metabolic engineering

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Energy (miscellaneous)
  • Renewable Energy, Sustainability and the Environment

Cite this

Detoxification of Lignocellulose Hydrolysates : Biochemical and Metabolic Engineering Toward White Biotechnology. / Chandel, Anuj K.; da Silva, Silvio Silvério; Singh, Om V.

In: Bioenergy Research, Vol. 6, No. 1, 2013, p. 388-401.

Research output: Contribution to journalArticle

Chandel, Anuj K. ; da Silva, Silvio Silvério ; Singh, Om V. / Detoxification of Lignocellulose Hydrolysates : Biochemical and Metabolic Engineering Toward White Biotechnology. In: Bioenergy Research. 2013 ; Vol. 6, No. 1. pp. 388-401.
@article{9f0fb838d9ce438191bd6c1c2d36bf59,
title = "Detoxification of Lignocellulose Hydrolysates: Biochemical and Metabolic Engineering Toward White Biotechnology",
abstract = "Chemical hydrolysis of lignocellulosic biomass (LB) produces a number of inhibitors in addition to sugars. These inhibitors include lignin-derived phenolics, carbohydrate-derived furans, and weak acids that have shown a marked effect on the productivities of various metabolites and the growth of biocatalysts in the fermentative reaction. In the past, a number of physicochemical and biological approaches have been proposed to overcome these fermentation inhibitors, including modified fermentative strategies. Additionally, the timely intervention of genetic engineering has provided an impetus to develop suitable technologies for the detoxification of lignocellulosics in biorefineries. However, the improvements in detoxification strategies for lignocellulose hydrolysates have resulted in significant loss of sugars after detoxification. Hydrolysis of myco-LB (LB after fungal pretreatment) has been recognized as a promising approach to avoid fermentation inhibitors and improve total sugar recovery. Biotechnological inventions have also made it possible to widen the range of suitable biocatalysts for biorefineries by microbial-routed induction of enzymatic expression for the elimination of inhibitors, eventually improving ethanol production from acid hydrolysates. This article aims to highlight the strategies that have been adopted to detoxify lignocellulosic hydrolysates and their effects on the chemical composition of the hydrolysates to improve the fermentability of lignocellulosics. In addition, genetic manipulation could widen the availability and variety of substrates and modify the metabolic routes to produce bioethanol or other value-added compounds in an efficient manner.",
keywords = "Bioethanol, d-Xylitol, Detoxification, Fermentation, Lignocellulose hydrolysis, Metabolic engineering",
author = "Chandel, {Anuj K.} and {da Silva}, {Silvio Silv{\'e}rio} and Singh, {Om V.}",
year = "2013",
doi = "10.1007/s12155-012-9241-z",
language = "English (US)",
volume = "6",
pages = "388--401",
journal = "Bioenergy Research",
issn = "1939-1234",
publisher = "Springer New York",
number = "1",

}

TY - JOUR

T1 - Detoxification of Lignocellulose Hydrolysates

T2 - Biochemical and Metabolic Engineering Toward White Biotechnology

AU - Chandel, Anuj K.

AU - da Silva, Silvio Silvério

AU - Singh, Om V.

PY - 2013

Y1 - 2013

N2 - Chemical hydrolysis of lignocellulosic biomass (LB) produces a number of inhibitors in addition to sugars. These inhibitors include lignin-derived phenolics, carbohydrate-derived furans, and weak acids that have shown a marked effect on the productivities of various metabolites and the growth of biocatalysts in the fermentative reaction. In the past, a number of physicochemical and biological approaches have been proposed to overcome these fermentation inhibitors, including modified fermentative strategies. Additionally, the timely intervention of genetic engineering has provided an impetus to develop suitable technologies for the detoxification of lignocellulosics in biorefineries. However, the improvements in detoxification strategies for lignocellulose hydrolysates have resulted in significant loss of sugars after detoxification. Hydrolysis of myco-LB (LB after fungal pretreatment) has been recognized as a promising approach to avoid fermentation inhibitors and improve total sugar recovery. Biotechnological inventions have also made it possible to widen the range of suitable biocatalysts for biorefineries by microbial-routed induction of enzymatic expression for the elimination of inhibitors, eventually improving ethanol production from acid hydrolysates. This article aims to highlight the strategies that have been adopted to detoxify lignocellulosic hydrolysates and their effects on the chemical composition of the hydrolysates to improve the fermentability of lignocellulosics. In addition, genetic manipulation could widen the availability and variety of substrates and modify the metabolic routes to produce bioethanol or other value-added compounds in an efficient manner.

AB - Chemical hydrolysis of lignocellulosic biomass (LB) produces a number of inhibitors in addition to sugars. These inhibitors include lignin-derived phenolics, carbohydrate-derived furans, and weak acids that have shown a marked effect on the productivities of various metabolites and the growth of biocatalysts in the fermentative reaction. In the past, a number of physicochemical and biological approaches have been proposed to overcome these fermentation inhibitors, including modified fermentative strategies. Additionally, the timely intervention of genetic engineering has provided an impetus to develop suitable technologies for the detoxification of lignocellulosics in biorefineries. However, the improvements in detoxification strategies for lignocellulose hydrolysates have resulted in significant loss of sugars after detoxification. Hydrolysis of myco-LB (LB after fungal pretreatment) has been recognized as a promising approach to avoid fermentation inhibitors and improve total sugar recovery. Biotechnological inventions have also made it possible to widen the range of suitable biocatalysts for biorefineries by microbial-routed induction of enzymatic expression for the elimination of inhibitors, eventually improving ethanol production from acid hydrolysates. This article aims to highlight the strategies that have been adopted to detoxify lignocellulosic hydrolysates and their effects on the chemical composition of the hydrolysates to improve the fermentability of lignocellulosics. In addition, genetic manipulation could widen the availability and variety of substrates and modify the metabolic routes to produce bioethanol or other value-added compounds in an efficient manner.

KW - Bioethanol

KW - d-Xylitol

KW - Detoxification

KW - Fermentation

KW - Lignocellulose hydrolysis

KW - Metabolic engineering

UR - http://www.scopus.com/inward/record.url?scp=84873736520&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84873736520&partnerID=8YFLogxK

U2 - 10.1007/s12155-012-9241-z

DO - 10.1007/s12155-012-9241-z

M3 - Article

VL - 6

SP - 388

EP - 401

JO - Bioenergy Research

JF - Bioenergy Research

SN - 1939-1234

IS - 1

ER -