Determination of trimethylsilyl meethylated nucleic acid bases in uring by gas-liquid chromatography

Duane B. Lakings, Charles W. Gehrke, T. Phillip Waalkes

Research output: Contribution to journalArticlepeer-review

Abstract

A method for the determination of the urinary excretion level of methylated nucleic acid bases by gas-liquid chromatography (GLC) has been developed. A clean-up procedure prior to GLC analysis consisted of hydrolysis, filtration, charcoal adsorption, and anion exchange. Studies to determine optimum derivatization conditions for conversion of the methylated bases to their trimethylsilyl derivatives and to evaluate GLC parameters and columns to obtain the best separation were conducted. Application of the method was shown by determining the excretion levels of methylated bases in urine normal controls and of patients with various types of malignancy.

Original languageEnglish (US)
Pages (from-to)69-81
Number of pages13
JournalJournal of Chromatography A
Volume116
Issue number1
DOIs
StatePublished - Jan 7 1976
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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