Determination of the spermicide nonoxynol-9 in vaginal lavage by high-performance liquid chromatography

Jason L. McPherson, James H. Nichols, Patricia Barditch-Crovo, Fayez M. Hamzeh

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


A sensitive normal-phase high-performance liquid chromatographic method using a bonded-phase aminosilica column has been developed for the measurement of the spermicide nonoxynol-9 in vaginal lavage fluid. The mean multiple correlation coefficient (r2) for nonoxynol-9 was 0.999 over the calibration range 3.125-50 μg/ml for the standards. Quality control samples measured at two different concentration levels gave intra-day precision values (coefficient of variation, C.V.) in the range of 0.61 to 1.63% and the intra-day accuracy values (mean relative error, M.R.E.) in the range of 0.13-0.62%. Inter-day precision and accuracy values from five different calibration standard concentration values ranged from 2.25 to 5.09% C.V. and 4.02 to 7.56% M.R.E. Nonoxynol-9 samples examined for peak area stability at room temperature over a 24-h time period had a M.R.E. of 14.9%. Quality control samples stored at 70°C, and tested after one month by comparison to baseline samples, had a M.R.E. of -10% and -7.53% for the low and high quality control samples, respectively. The method is sensitive and simple, with short runtimes, to enable the processing of numerous samples from a clinical trial.

Original languageEnglish (US)
Pages (from-to)204-208
Number of pages5
JournalJournal of Chromatography B: Biomedical Applications
Issue number1
StatePublished - Feb 23 1996
Externally publishedYes


  • Nonoxynol-9

ASJC Scopus subject areas

  • Chemistry(all)


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