TY - JOUR
T1 - Determination of the spermicide nonoxynol-9 in vaginal lavage by high-performance liquid chromatography
AU - McPherson, Jason L.
AU - Nichols, James H.
AU - Barditch-Crovo, Patricia
AU - Hamzeh, Fayez M.
PY - 1996/2/23
Y1 - 1996/2/23
N2 - A sensitive normal-phase high-performance liquid chromatographic method using a bonded-phase aminosilica column has been developed for the measurement of the spermicide nonoxynol-9 in vaginal lavage fluid. The mean multiple correlation coefficient (r2) for nonoxynol-9 was 0.999 over the calibration range 3.125-50 μg/ml for the standards. Quality control samples measured at two different concentration levels gave intra-day precision values (coefficient of variation, C.V.) in the range of 0.61 to 1.63% and the intra-day accuracy values (mean relative error, M.R.E.) in the range of 0.13-0.62%. Inter-day precision and accuracy values from five different calibration standard concentration values ranged from 2.25 to 5.09% C.V. and 4.02 to 7.56% M.R.E. Nonoxynol-9 samples examined for peak area stability at room temperature over a 24-h time period had a M.R.E. of 14.9%. Quality control samples stored at 70°C, and tested after one month by comparison to baseline samples, had a M.R.E. of -10% and -7.53% for the low and high quality control samples, respectively. The method is sensitive and simple, with short runtimes, to enable the processing of numerous samples from a clinical trial.
AB - A sensitive normal-phase high-performance liquid chromatographic method using a bonded-phase aminosilica column has been developed for the measurement of the spermicide nonoxynol-9 in vaginal lavage fluid. The mean multiple correlation coefficient (r2) for nonoxynol-9 was 0.999 over the calibration range 3.125-50 μg/ml for the standards. Quality control samples measured at two different concentration levels gave intra-day precision values (coefficient of variation, C.V.) in the range of 0.61 to 1.63% and the intra-day accuracy values (mean relative error, M.R.E.) in the range of 0.13-0.62%. Inter-day precision and accuracy values from five different calibration standard concentration values ranged from 2.25 to 5.09% C.V. and 4.02 to 7.56% M.R.E. Nonoxynol-9 samples examined for peak area stability at room temperature over a 24-h time period had a M.R.E. of 14.9%. Quality control samples stored at 70°C, and tested after one month by comparison to baseline samples, had a M.R.E. of -10% and -7.53% for the low and high quality control samples, respectively. The method is sensitive and simple, with short runtimes, to enable the processing of numerous samples from a clinical trial.
KW - Nonoxynol-9
UR - http://www.scopus.com/inward/record.url?scp=0029964071&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029964071&partnerID=8YFLogxK
U2 - 10.1016/0378-4347(95)00413-0
DO - 10.1016/0378-4347(95)00413-0
M3 - Article
C2 - 8925098
AN - SCOPUS:0029964071
SN - 1572-6495
VL - 677
SP - 204
EP - 208
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 1
ER -