TY - JOUR
T1 - Determination of the docetaxel vehicle, polysorbate 80, in patient samples by liquid chromatography-tandem mass spectrometry
AU - Sparreboom, Alex
AU - Zhao, Ming
AU - Brahmer, Julie R.
AU - Verweij, Jaap
AU - Baker, Sharyn D.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2002/6/25
Y1 - 2002/6/25
N2 - A new simple method was developed for the quantitative determination of the docetaxel (Taxotere) vehicle, polysorbate 80 (Tween 80), in human plasma. Calibration curves were constructed in the range of 1-100 μg/ml, using paclitaxel (0.01 mM) as internal standard, and were analyzed using a power fit with equal weighting. Sample pretreatment involved a one-step extraction with acetonitrile-n-butyl chloride (1:4, v/v). The analytes were separated on a Waters X-Terra MS column (50×2.1 mm I.D.) packed with 3.5-μm ODS material, and eluted with methanol-water (9:1, v/v) containing 0.1% formic acid. The column effluent was monitored by tandem mass spectrometry with electrospray ionization. The overall extraction efficiency was 50-60%, with values for precision and accuracy of ≤16% and <15% relative error, respectively. Our current method is ∼60-100-fold more sensitive than previous assays, and will be used to define Tween 80 disposition in patients receiving Taxotere.
AB - A new simple method was developed for the quantitative determination of the docetaxel (Taxotere) vehicle, polysorbate 80 (Tween 80), in human plasma. Calibration curves were constructed in the range of 1-100 μg/ml, using paclitaxel (0.01 mM) as internal standard, and were analyzed using a power fit with equal weighting. Sample pretreatment involved a one-step extraction with acetonitrile-n-butyl chloride (1:4, v/v). The analytes were separated on a Waters X-Terra MS column (50×2.1 mm I.D.) packed with 3.5-μm ODS material, and eluted with methanol-water (9:1, v/v) containing 0.1% formic acid. The column effluent was monitored by tandem mass spectrometry with electrospray ionization. The overall extraction efficiency was 50-60%, with values for precision and accuracy of ≤16% and <15% relative error, respectively. Our current method is ∼60-100-fold more sensitive than previous assays, and will be used to define Tween 80 disposition in patients receiving Taxotere.
KW - Docetaxel
KW - Polysorbate 80
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U2 - 10.1016/S1570-0232(02)00167-8
DO - 10.1016/S1570-0232(02)00167-8
M3 - Article
C2 - 12031845
AN - SCOPUS:0037172928
SN - 1570-0232
VL - 773
SP - 183
EP - 190
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 2
ER -