Determination of the docetaxel vehicle, polysorbate 80, in patient samples by liquid chromatography-tandem mass spectrometry

Alex Sparreboom, Ming Zhao, Julie R. Brahmer, Jaap Verweij, Sharyn D. Baker

Research output: Contribution to journalArticlepeer-review

Abstract

A new simple method was developed for the quantitative determination of the docetaxel (Taxotere) vehicle, polysorbate 80 (Tween 80), in human plasma. Calibration curves were constructed in the range of 1-100 μg/ml, using paclitaxel (0.01 mM) as internal standard, and were analyzed using a power fit with equal weighting. Sample pretreatment involved a one-step extraction with acetonitrile-n-butyl chloride (1:4, v/v). The analytes were separated on a Waters X-Terra MS column (50×2.1 mm I.D.) packed with 3.5-μm ODS material, and eluted with methanol-water (9:1, v/v) containing 0.1% formic acid. The column effluent was monitored by tandem mass spectrometry with electrospray ionization. The overall extraction efficiency was 50-60%, with values for precision and accuracy of ≤16% and <15% relative error, respectively. Our current method is ∼60-100-fold more sensitive than previous assays, and will be used to define Tween 80 disposition in patients receiving Taxotere.

Original languageEnglish (US)
Pages (from-to)183-190
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume773
Issue number2
DOIs
StatePublished - Jun 25 2002

Keywords

  • Docetaxel
  • Polysorbate 80

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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