Determination of testosterone and androst-4-ene-3,17-dione concentration in human plasma

Marco A. Rivarola, Claude J. Migeon

Research output: Contribution to journalArticlepeer-review


A double isotope dilution method is presented for the determination of testosterone and andros T-4-ene-3, 17-dione concentration in human plasma consisting of the main following steps: 1/ ether extraction and Florisil column chromatography; 2/ paper chromatography (System 1) for separation of testosterone and androst-4-ene-3, 17-dione; 3/ NaBH4 reduction of andros T-4-ene-3, 17-dione to testosterone and paper chromatography (System 11); 4/ acetylation of testosterone with C14-acetic anhydride; 5/ bidimensional thin layer chromatography; 6/ paper chromatography (System 111); 7/ NaBH4 reduction of testosterone acetate to 3β-hydroxy-androst-4-ene-17β-acetate; 8/ paper chromatography (System 1). After correction for the blank, mean testosterone and androst-4-ene-3,17-dione plasma concentrations were found to be 554 and 99 mμg/100 ml for normal adult males and 41 and 180 mμg/100 ml for normal adult females.

Original languageEnglish (US)
Pages (from-to)103-117
Number of pages15
Issue number2
StatePublished - Feb 1966

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology
  • Pharmacology
  • Clinical Biochemistry
  • Organic Chemistry


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