Determination of testosterone and androst-4-ene-3,17-dione concentration in human plasma

Marco A. Rivarola, Claude J. Migeon

Research output: Contribution to journalArticle

Abstract

A double isotope dilution method is presented for the determination of testosterone and andros T-4-ene-3, 17-dione concentration in human plasma consisting of the main following steps: 1/ ether extraction and Florisil column chromatography; 2/ paper chromatography (System 1) for separation of testosterone and androst-4-ene-3, 17-dione; 3/ NaBH4 reduction of andros T-4-ene-3, 17-dione to testosterone and paper chromatography (System 11); 4/ acetylation of testosterone with C14-acetic anhydride; 5/ bidimensional thin layer chromatography; 6/ paper chromatography (System 111); 7/ NaBH4 reduction of testosterone acetate to 3β-hydroxy-androst-4-ene-17β-acetate; 8/ paper chromatography (System 1). After correction for the blank, mean testosterone and androst-4-ene-3,17-dione plasma concentrations were found to be 554 and 99 mμg/100 ml for normal adult males and 41 and 180 mμg/100 ml for normal adult females.

Original languageEnglish (US)
Pages (from-to)103-117
Number of pages15
JournalSteroids
Volume7
Issue number2
StatePublished - Feb 1966

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Plasma (human)
Paper Chromatography
Androstenedione
Testosterone
Chromatography
Acetylation
Thin Layer Chromatography
Thin layer chromatography
Column chromatography
Isotopes
Ether
Acetates
Dilution
Plasmas

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology
  • Biochemistry

Cite this

Determination of testosterone and androst-4-ene-3,17-dione concentration in human plasma. / Rivarola, Marco A.; Migeon, Claude J.

In: Steroids, Vol. 7, No. 2, 02.1966, p. 103-117.

Research output: Contribution to journalArticle

Rivarola, MA & Migeon, CJ 1966, 'Determination of testosterone and androst-4-ene-3,17-dione concentration in human plasma', Steroids, vol. 7, no. 2, pp. 103-117.
Rivarola, Marco A. ; Migeon, Claude J. / Determination of testosterone and androst-4-ene-3,17-dione concentration in human plasma. In: Steroids. 1966 ; Vol. 7, No. 2. pp. 103-117.
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