Determination of testosterone and androst-4-ene-3,17-dione concentration in human plasma

Marco A. Rivarola; Claude J. Migeon

doi:10.1016/0039-128X(66)90019-5

Determination of testosterone and androst-4-ene-3,17-dione concentration in human plasma

Marco A. Rivarola, Claude J. Migeon

School of Medicine

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

A double isotope dilution method is presented for the determination of testosterone and andros T-4-ene-3, 17-dione concentration in human plasma consisting of the main following steps: 1/ ether extraction and Florisil column chromatography; 2/ paper chromatography (System 1) for separation of testosterone and androst-4-ene-3, 17-dione; 3/ NaBH₄ reduction of andros T-4-ene-3, 17-dione to testosterone and paper chromatography (System 11); 4/ acetylation of testosterone with C¹⁴-acetic anhydride; 5/ bidimensional thin layer chromatography; 6/ paper chromatography (System 111); 7/ NaBH₄ reduction of testosterone acetate to 3β-hydroxy-androst-4-ene-17β-acetate; 8/ paper chromatography (System 1). After correction for the blank, mean testosterone and androst-4-ene-3,17-dione plasma concentrations were found to be 55⁴ and 99 mμg/100 ml for normal adult males and 41 and 180 mμg/100 ml for normal adult females.

Original language	English (US)
Pages (from-to)	103-117
Number of pages	15
Journal	Steroids
Volume	7
Issue number	2
DOIs	https://doi.org/10.1016/0039-128X(66)90019-5
State	Published - Feb 1966

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Endocrinology
Pharmacology
Clinical Biochemistry
Organic Chemistry

Access to Document

10.1016/0039-128X(66)90019-5

Cite this

@article{b27de8bb1bff49d3849abf524e25929f,

title = "Determination of testosterone and androst-4-ene-3,17-dione concentration in human plasma",

abstract = "A double isotope dilution method is presented for the determination of testosterone and andros T-4-ene-3, 17-dione concentration in human plasma consisting of the main following steps: 1/ ether extraction and Florisil column chromatography; 2/ paper chromatography (System 1) for separation of testosterone and androst-4-ene-3, 17-dione; 3/ NaBH4 reduction of andros T-4-ene-3, 17-dione to testosterone and paper chromatography (System 11); 4/ acetylation of testosterone with C14-acetic anhydride; 5/ bidimensional thin layer chromatography; 6/ paper chromatography (System 111); 7/ NaBH4 reduction of testosterone acetate to 3β-hydroxy-androst-4-ene-17β-acetate; 8/ paper chromatography (System 1). After correction for the blank, mean testosterone and androst-4-ene-3,17-dione plasma concentrations were found to be 554 and 99 mμg/100 ml for normal adult males and 41 and 180 mμg/100 ml for normal adult females.",

author = "Rivarola, {Marco A.} and Migeon, {Claude J.}",

year = "1966",

month = feb,

doi = "10.1016/0039-128X(66)90019-5",

language = "English (US)",

volume = "7",

pages = "103--117",

journal = "Steroids",

issn = "0039-128X",

publisher = "Elsevier Inc.",

number = "2",

}

TY - JOUR

T1 - Determination of testosterone and androst-4-ene-3,17-dione concentration in human plasma

AU - Rivarola, Marco A.

AU - Migeon, Claude J.

PY - 1966/2

Y1 - 1966/2

N2 - A double isotope dilution method is presented for the determination of testosterone and andros T-4-ene-3, 17-dione concentration in human plasma consisting of the main following steps: 1/ ether extraction and Florisil column chromatography; 2/ paper chromatography (System 1) for separation of testosterone and androst-4-ene-3, 17-dione; 3/ NaBH4 reduction of andros T-4-ene-3, 17-dione to testosterone and paper chromatography (System 11); 4/ acetylation of testosterone with C14-acetic anhydride; 5/ bidimensional thin layer chromatography; 6/ paper chromatography (System 111); 7/ NaBH4 reduction of testosterone acetate to 3β-hydroxy-androst-4-ene-17β-acetate; 8/ paper chromatography (System 1). After correction for the blank, mean testosterone and androst-4-ene-3,17-dione plasma concentrations were found to be 554 and 99 mμg/100 ml for normal adult males and 41 and 180 mμg/100 ml for normal adult females.

AB - A double isotope dilution method is presented for the determination of testosterone and andros T-4-ene-3, 17-dione concentration in human plasma consisting of the main following steps: 1/ ether extraction and Florisil column chromatography; 2/ paper chromatography (System 1) for separation of testosterone and androst-4-ene-3, 17-dione; 3/ NaBH4 reduction of andros T-4-ene-3, 17-dione to testosterone and paper chromatography (System 11); 4/ acetylation of testosterone with C14-acetic anhydride; 5/ bidimensional thin layer chromatography; 6/ paper chromatography (System 111); 7/ NaBH4 reduction of testosterone acetate to 3β-hydroxy-androst-4-ene-17β-acetate; 8/ paper chromatography (System 1). After correction for the blank, mean testosterone and androst-4-ene-3,17-dione plasma concentrations were found to be 554 and 99 mμg/100 ml for normal adult males and 41 and 180 mμg/100 ml for normal adult females.

UR - http://www.scopus.com/inward/record.url?scp=0013882348&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0013882348&partnerID=8YFLogxK

U2 - 10.1016/0039-128X(66)90019-5

DO - 10.1016/0039-128X(66)90019-5

M3 - Article

C2 - 5922192

AN - SCOPUS:0013882348

SN - 0039-128X

VL - 7

SP - 103

EP - 117

JO - Steroids

JF - Steroids

IS - 2

ER -

Determination of testosterone and androst-4-ene-3,17-dione concentration in human plasma

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this