Determination of Ro 15-1788, a benzodiazepine antagonist, in human plasma by gas-liquid chromatography with nitrogen-phosphorus detection: Application to single-dose pharmacokinetic studies

Darrell R. Abernethy; Rainer M. Arendt; Peter M. Lauven; David J. Greenblatt

doi:10.1159/000137812

Determination of Ro 15-1788, a benzodiazepine antagonist, in human plasma by gas-liquid chromatography with nitrogen-phosphorus detection: Application to single-dose pharmacokinetic studies

Darrell R. Abernethy, Rainer M. Arendt, Peter M. Lauven, David J. Greenblatt

Research output: Contribution to journal › Article › peer-review

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Abstract

A sensitive (to 3 ng/ml) and specific method for analysis of the benzodiazepine antagonist Ro 15-1788 is described. The method utilizes gas-liquid chromatography with nitrogen-phosphorus detection. A neutral pH ethyl acetate extraction of 0.1–3.0 ml plasma is used for sample preparation. Standard curves using methylclonazepam as the internal standard are linear for plasma concentrations up to 200 ng/ml. Applicability of the method is demonstrated by a pharmacokinetic study in a normal volunteer who received 10 mg Ro 15-1788 intravenously.

Original language	English (US)
Pages (from-to)	285-289
Number of pages	5
Journal	Pharmacology
Volume	26
Issue number	5
DOIs	https://doi.org/10.1159/000137812
State	Published - 1983
Externally published	Yes

Keywords

Gas-liquid chromatography
Pharmacokinetics
Ro 15-1788

ASJC Scopus subject areas

Pharmacology

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10.1159/000137812

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Determination of Ro 15-1788, a benzodiazepine antagonist, in human plasma by gas-liquid chromatography with nitrogen-phosphorus detection: Application to single-dose pharmacokinetic studies. / Abernethy, Darrell R.; Arendt, Rainer M.; Lauven, Peter M. et al.
In: Pharmacology, Vol. 26, No. 5, 1983, p. 285-289.

Research output: Contribution to journal › Article › peer-review

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abstract = "A sensitive (to 3 ng/ml) and specific method for analysis of the benzodiazepine antagonist Ro 15-1788 is described. The method utilizes gas-liquid chromatography with nitrogen-phosphorus detection. A neutral pH ethyl acetate extraction of 0.1–3.0 ml plasma is used for sample preparation. Standard curves using methylclonazepam as the internal standard are linear for plasma concentrations up to 200 ng/ml. Applicability of the method is demonstrated by a pharmacokinetic study in a normal volunteer who received 10 mg Ro 15-1788 intravenously.",

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AU - Lauven, Peter M.

AU - Greenblatt, David J.

PY - 1983

Y1 - 1983

N2 - A sensitive (to 3 ng/ml) and specific method for analysis of the benzodiazepine antagonist Ro 15-1788 is described. The method utilizes gas-liquid chromatography with nitrogen-phosphorus detection. A neutral pH ethyl acetate extraction of 0.1–3.0 ml plasma is used for sample preparation. Standard curves using methylclonazepam as the internal standard are linear for plasma concentrations up to 200 ng/ml. Applicability of the method is demonstrated by a pharmacokinetic study in a normal volunteer who received 10 mg Ro 15-1788 intravenously.

AB - A sensitive (to 3 ng/ml) and specific method for analysis of the benzodiazepine antagonist Ro 15-1788 is described. The method utilizes gas-liquid chromatography with nitrogen-phosphorus detection. A neutral pH ethyl acetate extraction of 0.1–3.0 ml plasma is used for sample preparation. Standard curves using methylclonazepam as the internal standard are linear for plasma concentrations up to 200 ng/ml. Applicability of the method is demonstrated by a pharmacokinetic study in a normal volunteer who received 10 mg Ro 15-1788 intravenously.

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KW - Pharmacokinetics

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Determination of Ro 15-1788, a benzodiazepine antagonist, in human plasma by gas-liquid chromatography with nitrogen-phosphorus detection: Application to single-dose pharmacokinetic studies

Abstract

Keywords

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