Determination of N-(phosphonacetyl)-L-aspartic acid (PALA) in plasma and urine by high pressure liquid chromatography

J. Lankelma, P. G M Penders, A. Leyva, H. M. Pinedo

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Abstract

A rapid and sensitive determination of N-(phosphonacetyl)-L-aspartic acid (PALA) in plasma and urine using high pressure liquid chromatography is reported. A microparticulate, strong anion exchange column was used for the separation and PALA was detected by u.v. absorption at 205 nm. Interfering substances were removed from deproteinized plasma or from urine by a simple and quick clean-up procedure involving extraction with organic solvents. The detection limit for PALA was 5 x 10-7M and linear dynamic range was greater than 104. The assay time required was less than an hour. Plasma concentrations and urinary excretion of PALA were measured using this method in two patients treated with PALA. The ease and rapidity of this assay are advantages over reported methods and make possible close monitoring of the pharmacokinetics of this drug.

Original languageEnglish (US)
Pages (from-to)1483-1487
Number of pages5
JournalEuropean Journal of Cancer and Clinical Oncology
Volume16
Issue number11
StatePublished - 1980
Externally publishedYes

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ASJC Scopus subject areas

  • Oncology

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