Determination of myocardial and serum digoxin concentrations in children by specific and nonspecific assay methods

John G. Wagner, Macdonald Dick, Douglas M. Behrendt, Graham F. Lockwood, Ermelinda Sakmar, Paul Hees

Research output: Contribution to journalArticle

Abstract

After obtaining samples at open heart surgery, serum and right atrial digoxin concentrations were measured in 25 children by a nonspecific, direct radioimmunoassay method (NS) and by a specific method in which digoxin was separated from its metabolites by HPLC before radioimmunoassay was applied to the digoxin fraction (S). Digoxin was detectable by S assay (sensitivity 0.1 ng/g in 16 heart specimens and 22 serum samples. The mean and range of the SINS ratio was 0.74 (0.23 to 2.63) for serum and 0.81 (0.068 to 1.38) for atrial tissue. By NS assay the mean and range of the atriallserum ratio was 78.1 (2.4 to 340, n = 21) and by S assay the corresponding values were 100 (10.7 to 318, n = 15). A multiple linear regression indicated that 72.5% of the variance of the heart digoxin concentrations measured by S assay were accounted,for by the variables height, body weight, daily digoxin dose before operation, plasma digoxin concentration by S assay, and BUN.

Original languageEnglish (US)
Pages (from-to)577-584
Number of pages8
JournalClinical Pharmacology and Therapeutics
Volume33
Issue number5
StatePublished - May 1983
Externally publishedYes

Fingerprint

Digoxin
Serum
Radioimmunoassay
Blood Urea Nitrogen
Thoracic Surgery
Linear Models
High Pressure Liquid Chromatography
Body Weight

ASJC Scopus subject areas

  • Pharmacology

Cite this

Determination of myocardial and serum digoxin concentrations in children by specific and nonspecific assay methods. / Wagner, John G.; Dick, Macdonald; Behrendt, Douglas M.; Lockwood, Graham F.; Sakmar, Ermelinda; Hees, Paul.

In: Clinical Pharmacology and Therapeutics, Vol. 33, No. 5, 05.1983, p. 577-584.

Research output: Contribution to journalArticle

Wagner, John G. ; Dick, Macdonald ; Behrendt, Douglas M. ; Lockwood, Graham F. ; Sakmar, Ermelinda ; Hees, Paul. / Determination of myocardial and serum digoxin concentrations in children by specific and nonspecific assay methods. In: Clinical Pharmacology and Therapeutics. 1983 ; Vol. 33, No. 5. pp. 577-584.
@article{63721a3701a142aaab957e5acb2af9bd,
title = "Determination of myocardial and serum digoxin concentrations in children by specific and nonspecific assay methods",
abstract = "After obtaining samples at open heart surgery, serum and right atrial digoxin concentrations were measured in 25 children by a nonspecific, direct radioimmunoassay method (NS) and by a specific method in which digoxin was separated from its metabolites by HPLC before radioimmunoassay was applied to the digoxin fraction (S). Digoxin was detectable by S assay (sensitivity 0.1 ng/g in 16 heart specimens and 22 serum samples. The mean and range of the SINS ratio was 0.74 (0.23 to 2.63) for serum and 0.81 (0.068 to 1.38) for atrial tissue. By NS assay the mean and range of the atriallserum ratio was 78.1 (2.4 to 340, n = 21) and by S assay the corresponding values were 100 (10.7 to 318, n = 15). A multiple linear regression indicated that 72.5{\%} of the variance of the heart digoxin concentrations measured by S assay were accounted,for by the variables height, body weight, daily digoxin dose before operation, plasma digoxin concentration by S assay, and BUN.",
author = "Wagner, {John G.} and Macdonald Dick and Behrendt, {Douglas M.} and Lockwood, {Graham F.} and Ermelinda Sakmar and Paul Hees",
year = "1983",
month = "5",
language = "English (US)",
volume = "33",
pages = "577--584",
journal = "Clinical Pharmacology and Therapeutics",
issn = "0009-9236",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - Determination of myocardial and serum digoxin concentrations in children by specific and nonspecific assay methods

AU - Wagner, John G.

AU - Dick, Macdonald

AU - Behrendt, Douglas M.

AU - Lockwood, Graham F.

AU - Sakmar, Ermelinda

AU - Hees, Paul

PY - 1983/5

Y1 - 1983/5

N2 - After obtaining samples at open heart surgery, serum and right atrial digoxin concentrations were measured in 25 children by a nonspecific, direct radioimmunoassay method (NS) and by a specific method in which digoxin was separated from its metabolites by HPLC before radioimmunoassay was applied to the digoxin fraction (S). Digoxin was detectable by S assay (sensitivity 0.1 ng/g in 16 heart specimens and 22 serum samples. The mean and range of the SINS ratio was 0.74 (0.23 to 2.63) for serum and 0.81 (0.068 to 1.38) for atrial tissue. By NS assay the mean and range of the atriallserum ratio was 78.1 (2.4 to 340, n = 21) and by S assay the corresponding values were 100 (10.7 to 318, n = 15). A multiple linear regression indicated that 72.5% of the variance of the heart digoxin concentrations measured by S assay were accounted,for by the variables height, body weight, daily digoxin dose before operation, plasma digoxin concentration by S assay, and BUN.

AB - After obtaining samples at open heart surgery, serum and right atrial digoxin concentrations were measured in 25 children by a nonspecific, direct radioimmunoassay method (NS) and by a specific method in which digoxin was separated from its metabolites by HPLC before radioimmunoassay was applied to the digoxin fraction (S). Digoxin was detectable by S assay (sensitivity 0.1 ng/g in 16 heart specimens and 22 serum samples. The mean and range of the SINS ratio was 0.74 (0.23 to 2.63) for serum and 0.81 (0.068 to 1.38) for atrial tissue. By NS assay the mean and range of the atriallserum ratio was 78.1 (2.4 to 340, n = 21) and by S assay the corresponding values were 100 (10.7 to 318, n = 15). A multiple linear regression indicated that 72.5% of the variance of the heart digoxin concentrations measured by S assay were accounted,for by the variables height, body weight, daily digoxin dose before operation, plasma digoxin concentration by S assay, and BUN.

UR - http://www.scopus.com/inward/record.url?scp=0020630201&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020630201&partnerID=8YFLogxK

M3 - Article

C2 - 6839630

AN - SCOPUS:0020630201

VL - 33

SP - 577

EP - 584

JO - Clinical Pharmacology and Therapeutics

JF - Clinical Pharmacology and Therapeutics

SN - 0009-9236

IS - 5

ER -