Determination of Km for oxygen of nitric oxide synthase isoforms

Appavoo Rengasamy, Roger A. Johns

Research output: Contribution to journalArticlepeer-review

Abstract

Biosynthesis of nitric oxide (NO) requires L-arginine and molecular oxygen. Although the apparent Km values for L-arginine of NO synthase isoforms have been reported, the apparent Km values for oxygen are unknown. Low oxygen tension has been shown to attenuate NO synthase activity and NO-dependent vascular relaxation. We investigated the effect of different concentrations of oxygen on NO synthase activity of bovine brain, cultured bovine aortic endothelial cells and RAW 264.7 macrophages. The apparent Km values for oxygen were 23.2 ± 2.8, 7.7 ± 1.6 and 6.3 ± 0.9 μM for the brain, endothelial and macrophage NO synthases, respectively. This suggests that pathophysiological conditions involving a decrease in tissue oxygen concentration may attenuate NO production.

Original languageEnglish (US)
Pages (from-to)30-33
Number of pages4
JournalJournal of Pharmacology and Experimental Therapeutics
Volume276
Issue number1
StatePublished - Jan 1996
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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