Intoplicine, a benzopyrido-indole derivative, is a novel anticancer agent currently under phase I clinical evaluation. A selective, sensitive normal-phase high-performance liquid chromatographic (HPLC) assay with fluorescence detection, suitable for the determination of intoplicine in human plasma and whole blood, is described. The sample pretreatment involves a protein precipitation step with 2-propanol. The reported assay was validated, and the stability of the analyte in plasma, in whole blood and in the extraction fluid was investigated. The method has been implemented in a pharmacokinetic phase I clinical trial with intoplicine given as a 24-h intravenous infusion.
|Original language||English (US)|
|Number of pages||8|
|Journal||Journal of Chromatography B: Biomedical Sciences and Applications|
|State||Published - May 5 1993|
ASJC Scopus subject areas
- Clinical Biochemistry
- Molecular Medicine