Determination of intoplicine, a new antitumour drug, in human whole blood and plasma by normal-phase high-performance liquid chromatography with fluorescence detection

R. van Gijn, H. Rosing, W. W. ten Bokkel Huinink, O. van Tellingen, J. B. Vermorken, A. J M Liefting, R. Bruno, H. M. Pinedo, J. H. Beijnen

Research output: Contribution to journalArticle

Abstract

Intoplicine, a benzopyrido-indole derivative, is a novel anticancer agent currently under phase I clinical evaluation. A selective, sensitive normal-phase high-performance liquid chromatographic (HPLC) assay with fluorescence detection, suitable for the determination of intoplicine in human plasma and whole blood, is described. The sample pretreatment involves a protein precipitation step with 2-propanol. The reported assay was validated, and the stability of the analyte in plasma, in whole blood and in the extraction fluid was investigated. The method has been implemented in a pharmacokinetic phase I clinical trial with intoplicine given as a 24-h intravenous infusion.

Original languageEnglish (US)
Pages (from-to)299-306
Number of pages8
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume614
Issue number2
DOIs
StatePublished - May 5 1993
Externally publishedYes

Fingerprint

High performance liquid chromatography
Antineoplastic Agents
Blood
Fluorescence
High Pressure Liquid Chromatography
Plasmas
Assays
Plasma (human)
Clinical Trials, Phase I
Pharmacokinetics
2-Propanol
Intravenous Infusions
Derivatives
Fluids
Liquids
intoplicine
Proteins

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Determination of intoplicine, a new antitumour drug, in human whole blood and plasma by normal-phase high-performance liquid chromatography with fluorescence detection. / van Gijn, R.; Rosing, H.; ten Bokkel Huinink, W. W.; van Tellingen, O.; Vermorken, J. B.; Liefting, A. J M; Bruno, R.; Pinedo, H. M.; Beijnen, J. H.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 614, No. 2, 05.05.1993, p. 299-306.

Research output: Contribution to journalArticle

van Gijn, R. ; Rosing, H. ; ten Bokkel Huinink, W. W. ; van Tellingen, O. ; Vermorken, J. B. ; Liefting, A. J M ; Bruno, R. ; Pinedo, H. M. ; Beijnen, J. H. / Determination of intoplicine, a new antitumour drug, in human whole blood and plasma by normal-phase high-performance liquid chromatography with fluorescence detection. In: Journal of Chromatography B: Biomedical Sciences and Applications. 1993 ; Vol. 614, No. 2. pp. 299-306.
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AU - van Tellingen, O.

AU - Vermorken, J. B.

AU - Liefting, A. J M

AU - Bruno, R.

AU - Pinedo, H. M.

AU - Beijnen, J. H.

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