Abstract
Intoplicine, a benzopyrido-indole derivative, is a novel anticancer agent currently under phase I clinical evaluation. A selective, sensitive normal-phase high-performance liquid chromatographic (HPLC) assay with fluorescence detection, suitable for the determination of intoplicine in human plasma and whole blood, is described. The sample pretreatment involves a protein precipitation step with 2-propanol. The reported assay was validated, and the stability of the analyte in plasma, in whole blood and in the extraction fluid was investigated. The method has been implemented in a pharmacokinetic phase I clinical trial with intoplicine given as a 24-h intravenous infusion.
Original language | English (US) |
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Pages (from-to) | 299-306 |
Number of pages | 8 |
Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
Volume | 614 |
Issue number | 2 |
DOIs | |
State | Published - May 5 1993 |
Externally published | Yes |
ASJC Scopus subject areas
- General Chemistry
- Clinical Biochemistry
- Molecular Medicine