Determination of in vivo target search kinetics of regulatory noncoding RNA

Jingyi Fei, Digvijay Singh, Qiucen Zhang, Seongjin Park, Divya Balasubramanian, Ido Golding, Carin K. Vanderpool, Taekjip Ha

Research output: Contribution to journalArticle

Abstract

Base-pairing interactions between nucleic acids mediate target recognition in many biological processes.We developed a super-resolution imaging and modeling platform that enabled the in vivo determination of base pairing-mediated target recognition kinetics.We examined a stress-induced bacterial small RNA, SgrS, which induces the degradation of target messenger RNAs (mRNAs). SgrS binds to a primary target mRNA in a reversible and dynamic fashion, and formation of SgrS-mRNA complexes is rate-limiting, dictating the overall regulation efficiency in vivo. Examination of a secondary target indicated that differences in the target search kinetics contribute to setting the regulation priority among different target mRNAs.This super-resolution imaging and analysis approach provides a conceptual framework that can be generalized to other small RNA systems and other target search processes.

Original languageEnglish (US)
Pages (from-to)1371-1374
Number of pages4
JournalScience
Volume347
Issue number6228
DOIs
StatePublished - Mar 20 2015
Externally publishedYes

ASJC Scopus subject areas

  • General

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    Fei, J., Singh, D., Zhang, Q., Park, S., Balasubramanian, D., Golding, I., Vanderpool, C. K., & Ha, T. (2015). Determination of in vivo target search kinetics of regulatory noncoding RNA. Science, 347(6228), 1371-1374. https://doi.org/10.1126/science.1258849