Determination of in vivo target search kinetics of regulatory noncoding RNA

Jingyi Fei, Digvijay Singh, Qiucen Zhang, Seongjin Park, Divya Balasubramanian, Ido Golding, Carin K. Vanderpool, Taekjip Ha

Research output: Contribution to journalArticle

Abstract

Base-pairing interactions between nucleic acids mediate target recognition in many biological processes.We developed a super-resolution imaging and modeling platform that enabled the in vivo determination of base pairing-mediated target recognition kinetics.We examined a stress-induced bacterial small RNA, SgrS, which induces the degradation of target messenger RNAs (mRNAs). SgrS binds to a primary target mRNA in a reversible and dynamic fashion, and formation of SgrS-mRNA complexes is rate-limiting, dictating the overall regulation efficiency in vivo. Examination of a secondary target indicated that differences in the target search kinetics contribute to setting the regulation priority among different target mRNAs.This super-resolution imaging and analysis approach provides a conceptual framework that can be generalized to other small RNA systems and other target search processes.

Original languageEnglish (US)
Pages (from-to)1371-1374
Number of pages4
JournalScience
Volume347
Issue number6228
DOIs
StatePublished - Mar 20 2015
Externally publishedYes

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Untranslated RNA
Messenger RNA
Base Pairing
Bacterial RNA
Biological Phenomena
Nucleic Acids
RNA

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

Fei, J., Singh, D., Zhang, Q., Park, S., Balasubramanian, D., Golding, I., ... Ha, T. (2015). Determination of in vivo target search kinetics of regulatory noncoding RNA. Science, 347(6228), 1371-1374. https://doi.org/10.1126/science.1258849

Determination of in vivo target search kinetics of regulatory noncoding RNA. / Fei, Jingyi; Singh, Digvijay; Zhang, Qiucen; Park, Seongjin; Balasubramanian, Divya; Golding, Ido; Vanderpool, Carin K.; Ha, Taekjip.

In: Science, Vol. 347, No. 6228, 20.03.2015, p. 1371-1374.

Research output: Contribution to journalArticle

Fei, J, Singh, D, Zhang, Q, Park, S, Balasubramanian, D, Golding, I, Vanderpool, CK & Ha, T 2015, 'Determination of in vivo target search kinetics of regulatory noncoding RNA', Science, vol. 347, no. 6228, pp. 1371-1374. https://doi.org/10.1126/science.1258849
Fei J, Singh D, Zhang Q, Park S, Balasubramanian D, Golding I et al. Determination of in vivo target search kinetics of regulatory noncoding RNA. Science. 2015 Mar 20;347(6228):1371-1374. https://doi.org/10.1126/science.1258849
Fei, Jingyi ; Singh, Digvijay ; Zhang, Qiucen ; Park, Seongjin ; Balasubramanian, Divya ; Golding, Ido ; Vanderpool, Carin K. ; Ha, Taekjip. / Determination of in vivo target search kinetics of regulatory noncoding RNA. In: Science. 2015 ; Vol. 347, No. 6228. pp. 1371-1374.
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