Determination of in-gel protein concentration by a ninhydrin-based method

Sung Ung Kang, Gert Lubec

Research output: Contribution to journalArticlepeer-review

Abstract

Until now quantification of proteins in gel-based methods relies on the amount of protein loaded onto the gel. This does not, however, represent the amount of proteins in the gel and therefore determination of proteins within the gel is mandatory. A method to quantify proteins, both hydrophilic and hydrophobic, using in-gel digestion with proteases, subsequent acid hydrolysis and the use of the ninhydrin reaction is herein presented.

Original languageEnglish (US)
Pages (from-to)481-484
Number of pages4
JournalProteomics
Volume11
Issue number3
DOIs
StatePublished - Feb 2011
Externally publishedYes

Keywords

  • Hydrophobic protein
  • Ninhydrin assay
  • Protein quantification
  • Technology

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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