Determination Of Glycerol And Serum Triglycerides With Collagen-Immobilized Glycerol Dehydrogenase And Amperometric Detection

Tekum Fonong

Research output: Contribution to journalArticlepeer-review

Abstract

An amperometric procedure is described for the determination of glycerol and triglycerides in aqueous samples and in serum using glycerol dehydrogenase immobilized on a collagen membrane. Glycerol is determined by measurement of the steady-state oxidation currents generated at a platinum electrode by NADH produced in the enzyme-catalyzed reaction. The triglycerides were first hydrolyzed by the enzyme lipase in solution and the resulting glycerol determined similarly. Olive oil, determined to contain 78 % triolein, was used as the source of triglycerides in this study. For both glycerol and triglycerides the calibration plots are linear in the range from 0 to 12 μM, with detection limits of 0.2 and 0.7 μM, respectively. The immobilized glycerol dehydrogenase retained high operational activity for a period longer than 30 days.

Original languageEnglish (US)
Pages (from-to)783-796
Number of pages14
JournalAnalytical Letters
Volume20
Issue number5
DOIs
StatePublished - May 1987

Keywords

  • Glycerol
  • amperometric detection
  • immobilized glycerol dehydrogenase
  • olive oil
  • serum triglycerides

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

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